Mfg Part Number
Ab02895-15.0
Vector Labs Ab02895-15.0 Anti-AC toxin [2F5], Human IgM, lambda (50 μg)
Quick Overview
The mouse version of this antibody was generated by immunizing BALB/c mice with Bordetella pertussis adenylate cyclase toxin purified using sucrose gradient centrifugation.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 2F5 |
| Synonyms: | AC; Bordetella pertussis adenylate cyclase toxin; adenylate cyclase toxin |
| Amount: | 50 μg |
| Application Codes Clone: | WB; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the glycine and aspartate-rich nonapeptide repeat region (amino acids 1156-1489) of Bordetella pertussis adenylate cyclase toxin. |
| Application Notes: | The mouse version of this antibody was used for immunoblotting on different epitopes of the adenylate cyclase toxin from Bordetella pertussis. It was most sensitive to amino acids 1156-1489 (Lee et al, 1999; pmid:10225859). An ELISA was preformed on purified AC toxin using the mouse version of this antibody. The binding of the mouse version of this antibody was assessed at different calcium concentrations. It showed only a small decrease in binding affinity at high calcium concentration (Hewlett et al, 1991; pmid:1894634). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |