Vector Labs Ab02896-23.0 Anti-MAP-2 [AA5 (AP18)], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: AA5 (AP18)
Synonyms: Methionine aminopeptidase 2; MetAP 2; MAP 2; Initiation factor 2-associated 67 kDa glycoprotein; p67; p67eIF2
Amount: 200 μg
Application Codes Clone: WB; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific to the phosphorylated form of MAP2a, b, c and shows no cross-reactivity with other MAPs, tau or tubulin. This antibody reacts with dendrites and cell bodies of neurons. It is useful in studies of neuron structure in normal and malignant brain tissue and in Alzheimer's. MAP-2 cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged.
Application Notes: While testing the specificity of the mouse version of this antibody, a western blot on P9 quail and adult rat brain was performed using the mouse version of this antibody. Furthermore, quail cerebellum was immunologically stained using the mouse version of this antibody (Tucker et al, 1988; pmid:3199190). To examine the relationship between changes in tau phosphorylation and loss of MAP2 immunmoreactivity, immunocytochemistry was performed (Ashford et al, 1998; pmid:9786247). While assessing experience-dependent modifications in MAP2 phosphorylation in rat olfactory bulb. a western blot was performed against adult rat olfactory bulb proteins using the mouse version of this antibody. Furthermore, immunohistochemistry was performed against coronal sections of olfactory bulbs using the mouse version of this antibody (Philpot et al, 1997; pmid:9391014). While studying subependymal giant cell astrocytoma, immunohistochemistry was preformed using the mouse version of this antibody (Lopes et al, 1995; pmid:8928613).

Product Class:	Purified
Clone ID:	AA5 (AP18)
Synonyms:	Methionine aminopeptidase 2; MetAP 2; MAP 2; Initiation factor 2-associated 67 kDa glycoprotein; p67; p67eIF2
Amount:	200 μg
Application Codes Clone:	WB; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific to the phosphorylated form of MAP2a, b, c and shows no cross-reactivity with other MAPs, tau or tubulin. This antibody reacts with dendrites and cell bodies of neurons. It is useful in studies of neuron structure in normal and malignant brain tissue and in Alzheimer's. MAP-2 cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged.
Application Notes:	While testing the specificity of the mouse version of this antibody, a western blot on P9 quail and adult rat brain was performed using the mouse version of this antibody. Furthermore, quail cerebellum was immunologically stained using the mouse version of this antibody (Tucker et al, 1988; pmid:3199190). To examine the relationship between changes in tau phosphorylation and loss of MAP2 immunmoreactivity, immunocytochemistry was performed (Ashford et al, 1998; pmid:9786247). While assessing experience-dependent modifications in MAP2 phosphorylation in rat olfactory bulb. a western blot was performed against adult rat olfactory bulb proteins using the mouse version of this antibody. Furthermore, immunohistochemistry was performed against coronal sections of olfactory bulbs using the mouse version of this antibody (Philpot et al, 1997; pmid:9391014). While studying subependymal giant cell astrocytoma, immunohistochemistry was preformed using the mouse version of this antibody (Lopes et al, 1995; pmid:8928613).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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