Vector Labs Ab02905-6.1 Anti-beta tubulin type III [5G8], Rat IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 5G8
Synonyms: Tubulin beta-3 chain; beta tubulin type III; TUBB3; Tubulin beta-4 chain; Tubulin beta-III
Amount: 200 μg
Application Codes Clone: WB; IF; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for amino acids 436-450 of beta tubulin type III. IT binds to human, mice and rat beta tubulin type III. Its exact epitope is EAQGPK. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance.
Application Notes: The mouse version of this antibody was used for a western blot on purified beta tubulin type III (Spano and Frankfurter, 2010; pmid:20466128). Neuronal cultures of leucine-rich repeat kinase 2 knock-out mice were immunostained using the mouse version of this antibody (Gillardon, 2009; pmid:19545277). To determine selective expression of beta-tubulin isotypes in taxane resistance, immunohistochemistry was preformed on human primary ovarian carcinoma tissues using the mouse version of this antibody (Ohishi et al, 2007; pmid:17343904). While determining the localization of βIII-tubulin in colorectal cancer specimens, the mouse version of this antibody was used for immunohistochemistry on colorectal cancer tissue (Portyanko et al, 2009; pmid:19360438). To investigate the protein antigens targeted by antibody diversity of infected mice with Trypanosoma cruzi, immunoblots were preformed against recombinant TcβTUB (Montalvão et al, 2018; pmid:29706955).

Product Class:	Purified
Clone ID:	5G8
Synonyms:	Tubulin beta-3 chain; beta tubulin type III; TUBB3; Tubulin beta-4 chain; Tubulin beta-III
Amount:	200 μg
Application Codes Clone:	WB; IF; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for amino acids 436-450 of beta tubulin type III. IT binds to human, mice and rat beta tubulin type III. Its exact epitope is EAQGPK. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance.
Application Notes:	The mouse version of this antibody was used for a western blot on purified beta tubulin type III (Spano and Frankfurter, 2010; pmid:20466128). Neuronal cultures of leucine-rich repeat kinase 2 knock-out mice were immunostained using the mouse version of this antibody (Gillardon, 2009; pmid:19545277). To determine selective expression of beta-tubulin isotypes in taxane resistance, immunohistochemistry was preformed on human primary ovarian carcinoma tissues using the mouse version of this antibody (Ohishi et al, 2007; pmid:17343904). While determining the localization of βIII-tubulin in colorectal cancer specimens, the mouse version of this antibody was used for immunohistochemistry on colorectal cancer tissue (Portyanko et al, 2009; pmid:19360438). To investigate the protein antigens targeted by antibody diversity of infected mice with Trypanosoma cruzi, immunoblots were preformed against recombinant TcβTUB (Montalvão et al, 2018; pmid:29706955).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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