Vector Labs Ab02910-10.3 Anti-pp60Src [GD11], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: GD11
Heavy Chain Modification: Fc Silent™
Synonyms: Proto-oncogene tyrosine-protein kinase Src; SRC; Proto-oncogene c-Src; pp60c-src; p60-Src
Amount: 200 μg
Application Codes Clone: WB; IP; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for pp60src. It recognizes this protein in avians, humans and different rodents. It recognizes amino acids 82-169. Pp60src is a non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors.
Application Notes: To confirm wether a 60,000-Mr phosphoprotein was pp60src, an immunoprecipitation was preformed using the mouse version of this antibody. Furthermore, PrARSV-infected CE cells were immunostained using this antibody (Parsons et al, 1984; pmid:6205164). To determine wether pp60src is present in human T cells, an immunoprecipitation and subsequent western blot were preformed using the mouse version of this antibody on human T cell cultures (Branch and Mills, 1995; pmid:7535811). Proteins from fetal rat lung cells were immunoblotted using the mouse version of this antibody (Liu et al, 1996; pmid:8636139). While researching store-mediated Ca2+ entry, a western blot was preformed on human blood cells using the mouse version of this antibody (Redondo et al, 2005; pmid:15894173).

Product Class:	Purified
Clone ID:	GD11
Heavy Chain Modification:	Fc Silent™
Synonyms:	Proto-oncogene tyrosine-protein kinase Src; SRC; Proto-oncogene c-Src; pp60c-src; p60-Src
Amount:	200 μg
Application Codes Clone:	WB; IP; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for pp60src. It recognizes this protein in avians, humans and different rodents. It recognizes amino acids 82-169. Pp60src is a non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors.
Application Notes:	To confirm wether a 60,000-Mr phosphoprotein was pp60src, an immunoprecipitation was preformed using the mouse version of this antibody. Furthermore, PrARSV-infected CE cells were immunostained using this antibody (Parsons et al, 1984; pmid:6205164). To determine wether pp60src is present in human T cells, an immunoprecipitation and subsequent western blot were preformed using the mouse version of this antibody on human T cell cultures (Branch and Mills, 1995; pmid:7535811). Proteins from fetal rat lung cells were immunoblotted using the mouse version of this antibody (Liu et al, 1996; pmid:8636139). While researching store-mediated Ca2+ entry, a western blot was preformed on human blood cells using the mouse version of this antibody (Redondo et al, 2005; pmid:15894173).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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