Mfg Part Number
Ab02913-23.0

Vector Labs Ab02913-23.0 Anti-calf thymus ATPaseA [4B10-A3-E4], Rabbit IgG, kappa (200 μg)

Quick Overview
The mouse version of this antibody was generated by immunizing mice with immunopurified calf thymus ATPaseA.
$517.40
Order will ship within 1-3 business days
Add to Wish List
Product Class:Purified
Clone ID:4B10-A3-E4
Synonyms:SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein; Sucrose nonfermenting protein 2-like 1
Amount:200 μg
Application Codes Clone:WB; ELISA; functional assay
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for calf thymus ATPase A and recognizes bovine, human, and mouse homologs. Calf ATPaseA is a TP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. The antibody also shows a reaction to the denatured protein.
Application Notes:The mouse version of this antibody was used for a western blot on calf DNA-dependent ATPase A isolated from calf thymus using immunoprecipitation (Mesner et al, 1993; pmid:8394114). To identify the mouse version of this antibody an ELISA on native ATPase A was preformed. Furthermore, a western blot was preformed on bacteriophage T4 gene 44/62 and 45 proteins. It showed a strong reaction (Mesner et al, 1991; pmid:1720977). The ATPase A activity under either Ca2+-activated or Mg3+-actin-activated conditions were determined in the presence of this antibody. No change in activity was noticed (Eldin et al, 1993; pmid:8448113).
More Information
Brand Vector Labs
UNSPSC 12161500