Mfg Part Number
Ab02914-3.3
Vector Labs Ab02914-3.3 Anti-NTF2 [4F5], Mouse IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
The mouse version of this antibody was raised by immunizing mice with human NTF2.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 4F5 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Nuclear transport factor 2; NUTF2;Placental protein 15; PP15 |
| Amount: | 200 μg |
| Application Codes Clone: | WB; ELISA; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for NTF2. Its reactivity in humans, mice, rats, monkeys, hamsters, chickens and xenopus have been proven. The epitope of this antibody lies in the extreme C terminus of the NTF2 protein. NTF2 mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. |
| Application Notes: | To characterize this antibody, a western blot on recombinant NTF2 protein was preformed using the mouse version of this antibody. Further, an ELISA on wild-type and C-terminal truncation mutants was preformed using this antibody. It only detected the wild-type protein not the C-terminal truncated proteins. Finally, the distribution of ran was determined in living BHK21 cells in the presence of this antibody. Normally ran is nuclear but with this antibody ran is redistributed to the cytoplasm (Steggerda et al, 2000; pmid:10679025). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |