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Mfg Part Number

Ab02919-1.1

Vector Labs Ab02919-1.1 Anti-Frizzled [1C11-B9-H9-G9-D9], Mouse IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The mouse version of this antibody was generated by immunizing a mouse with amino acids 1-252 of Drosophila Frizzled.

$517.40

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Description

Product Class: Purified
Clone ID: 1C11-B9-H9-G9-D9
Synonyms: Frizzled protein; fz; Frizzled-1; dFz1
Amount: 200 μg
Application Codes Clone: IP; WB; IF; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for drosophila frizzled. It is specific for amino acids 1-252. Frizzled is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.
Application Notes: To study the biochemical characteristics of the frizzled protein. Proteins from schneider 2 cells were immunoprecipitated using the mouse version of this antibody. Futhermore the precipitates were immunoblotted using this antibody. Finally both schneider cells and pupal wings were immunostained using this antibody (Park et al, 1994; pmid:8199049). Protein lysates from drosophila L cells were immunoblotted using the mouse version of this antibody (Pradhan-Sundd and Verheyen, 2015; pmid:26224310). Adult drosophila wings were immunostained using the mouse version of this antibody (Adler et al, 1997; pmid:9382848). To determine the link between the wingless and the frizzled proteins. A immunolabeling was preformed on drosophila embryos using this antibody (Müller et al, 1999; pmid:9876186). To understand the wingless pathway. P19 cell lysates were immunoblotted using this antibody (Abdul-Ghani et al, 2011; pmid:21041481).

Product Class:	Purified
Clone ID:	1C11-B9-H9-G9-D9
Synonyms:	Frizzled protein; fz; Frizzled-1; dFz1
Amount:	200 μg
Application Codes Clone:	IP; WB; IF; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for drosophila frizzled. It is specific for amino acids 1-252. Frizzled is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.
Application Notes:	To study the biochemical characteristics of the frizzled protein. Proteins from schneider 2 cells were immunoprecipitated using the mouse version of this antibody. Futhermore the precipitates were immunoblotted using this antibody. Finally both schneider cells and pupal wings were immunostained using this antibody (Park et al, 1994; pmid:8199049). Protein lysates from drosophila L cells were immunoblotted using the mouse version of this antibody (Pradhan-Sundd and Verheyen, 2015; pmid:26224310). Adult drosophila wings were immunostained using the mouse version of this antibody (Adler et al, 1997; pmid:9382848). To determine the link between the wingless and the frizzled proteins. A immunolabeling was preformed on drosophila embryos using this antibody (Müller et al, 1999; pmid:9876186). To understand the wingless pathway. P19 cell lysates were immunoblotted using this antibody (Abdul-Ghani et al, 2011; pmid:21041481).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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