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Mfg Part Number

Ab02926-10.3

Vector Labs Ab02926-10.3 Anti-MCF-7 breast cancer cells [C3A8], Human IgG1, Fc Silent™, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing BALB/c mice with the MCF-7 breast carcinoma cell line.

$570.70

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Description

Product Class: Purified
Clone ID: C3A8
Heavy Chain Modification: Fc Silent™
Synonyms: MCF-7; MCF7; breast cancer cells
Amount: 100 μg
Application Codes Clone: ELISA; IF; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds an epitope found on the MCF-7 breast carcinoma cell line.
Application Notes: The original IgM version of this antibody strongly reacted with breast cancer cells (MCF7) and colon cancer cells (HT29) in an ELISA. Immunofluorescence staining suggested that the antigen this antibody detected was located in the cytoplasm of MCF7 and HT29 cell lines but there was no positive staining detected on cervical cancer (HeLa) and fibroblast normal cells (3T3). The original IgM version of the antibody detected a 55 kDa protein that was present in the extract of MCF7 and HT29 cell lines in a western blot assay (Abdullah & Ali., 2006). The scFv version of this antibody (B7) also showed high reactivity towards MCF-7 breast cancer cells in an ELISA (PMID: 24301925). The binding reactivity of the scFv version of this antibody was also tested using flow cytometry and it was suggested that the antigen was expressed in periplasmic space of MCF-7 cells when tested using an annexin V-based apoptosis assay (PMID: 25022376).

Product Class:	Purified
Clone ID:	C3A8
Heavy Chain Modification:	Fc Silent™
Synonyms:	MCF-7; MCF7; breast cancer cells
Amount:	100 μg
Application Codes Clone:	ELISA; IF; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds an epitope found on the MCF-7 breast carcinoma cell line.
Application Notes:	The original IgM version of this antibody strongly reacted with breast cancer cells (MCF7) and colon cancer cells (HT29) in an ELISA. Immunofluorescence staining suggested that the antigen this antibody detected was located in the cytoplasm of MCF7 and HT29 cell lines but there was no positive staining detected on cervical cancer (HeLa) and fibroblast normal cells (3T3). The original IgM version of the antibody detected a 55 kDa protein that was present in the extract of MCF7 and HT29 cell lines in a western blot assay (Abdullah & Ali., 2006). The scFv version of this antibody (B7) also showed high reactivity towards MCF-7 breast cancer cells in an ELISA (PMID: 24301925). The binding reactivity of the scFv version of this antibody was also tested using flow cytometry and it was suggested that the antigen was expressed in periplasmic space of MCF-7 cells when tested using an annexin V-based apoptosis assay (PMID: 25022376).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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