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Mfg Part Number

Ab02930-1.1

Vector Labs Ab02930-1.1 Anti-Zearalenone [QY1.5], Mouse IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated using phage display technology by panning an scFv library against zearalenone-ovalbumin conjugate. The scFv library was created by linking the heavy and light chain genes of antizearalenone hybridoma cell line (2G3-6E3-2E2) using DNA linker encoding peptide (Gly4Ser)3 and cloning into a phagemid (pCANTAB5E) to be expressed as a fusion protein on the surfaces of recombinant phages. The original hybridoma (2G3-6E3-2E2) was generated using zearalenone-BSA conjugate as an immunogen.

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Description

Product Class: Purified
Clone ID: QY1.5
Synonyms: RAL; F-2 toxin; F-2 mycotoxin; Mycotoxin F2; [6-(10-hydroxy-6-oxo-trans-1-undecenyl)-bresorcylic acid lactone]; CAS NO: 17924-92-4; ChEBI:10106
Amount: 200 μg
Application Codes Clone: ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody is specific for zearalenone and show some cross reactivity with hydroxylated zearalenone analogs. It is a mycotoxin produced mainly by fungi belonging to the genus Fusarium in foods and feeds. Possess estrogenic activity in pigs, cattle and sheep, with low acute toxicity. When fed to animals, the compound causes hypoestrogenism with symptoms such as enlargement of the uterus and nipples, vulvar swelling, vaginal prolapse, and infertility
Application Notes: The binding specificity of this scFv antibody towards mycotoxin zearalenone was determined using ELISA. The scFv version of this antibody was also able to detect zearalenone in spiked corn extract in a competitive-indirect ELISA (PMID: 8979354).

Product Class:	Purified
Clone ID:	QY1.5
Synonyms:	RAL; F-2 toxin; F-2 mycotoxin; Mycotoxin F2; [6-(10-hydroxy-6-oxo-trans-1-undecenyl)-bresorcylic acid lactone]; CAS NO: 17924-92-4; ChEBI:10106
Amount:	200 μg
Application Codes Clone:	ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody is specific for zearalenone and show some cross reactivity with hydroxylated zearalenone analogs. It is a mycotoxin produced mainly by fungi belonging to the genus Fusarium in foods and feeds. Possess estrogenic activity in pigs, cattle and sheep, with low acute toxicity. When fed to animals, the compound causes hypoestrogenism with symptoms such as enlargement of the uterus and nipples, vulvar swelling, vaginal prolapse, and infertility
Application Notes:	The binding specificity of this scFv antibody towards mycotoxin zearalenone was determined using ELISA. The scFv version of this antibody was also able to detect zearalenone in spiked corn extract in a competitive-indirect ELISA (PMID: 8979354).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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