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Mfg Part Number

Ab02940-23.0

Vector Labs Ab02940-23.0 Anti-Alpha-enolase [EnL5], Rabbit IgG, lambda (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing female white leghorn (Gallus domesticus) chickens with 100 μg purified α-enolase in an equal volume of Freund's complete adjuvant by an intramuscular injection. The total RNA was extracted from the enlarged spleens for scFv antibody library construction. This clone was isolated by panning the scFv library against alpha-enolase.

$517.40

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Description

Product Class: Purified
Clone ID: EnL5
Synonyms: α-enolase; MBP-1; MPB-1; ENO1; NNE; ENO1L1; MBPB1; MPB1; Enolase 1; Non-neural enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Phosphopyruvate hydratase; Plasminogen-binding protein
Amount: 200 μg
Application Codes Clone: ELISA; FC; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Chicken IgG format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds alpha-enolase, which is a glycolytic enzyme expressed in most tissues. It catalysis the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, it is involved in various processes such as growth control, hypoxia tolerance and allergic responses
Application Notes: The binding specificity of this antibody for alpha-enolase was done using ELISA. This antibody was also used for detection of α-enolase expressed in the PE089 cells using flow cytometry. This antibody was also used for immunofluorescent and immunocytochemical staining of α-enolase molecules expressed in PE089 cells (PMID: 20655599).

Product Class:	Purified
Clone ID:	EnL5
Synonyms:	α-enolase; MBP-1; MPB-1; ENO1; NNE; ENO1L1; MBPB1; MPB1; Enolase 1; Non-neural enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Phosphopyruvate hydratase; Plasminogen-binding protein
Amount:	200 μg
Application Codes Clone:	ELISA; FC; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Chicken IgG format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds alpha-enolase, which is a glycolytic enzyme expressed in most tissues. It catalysis the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, it is involved in various processes such as growth control, hypoxia tolerance and allergic responses
Application Notes:	The binding specificity of this antibody for alpha-enolase was done using ELISA. This antibody was also used for detection of α-enolase expressed in the PE089 cells using flow cytometry. This antibody was also used for immunofluorescent and immunocytochemical staining of α-enolase molecules expressed in PE089 cells (PMID: 20655599).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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