Mfg Part Number
Ab02944-23.0
Vector Labs Ab02944-23.0 Anti-Flavocytochrome B2 [B2B4], Rabbit IgG, lambda (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with fully purified Flb.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | B2B4 |
| Synonyms: | Cytochrome b2 mitochondrial; L-lactate dehydrogenase [Cytochrome]; L-lactate ferricytochrome C oxidoreductase; L-LCR |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody recognized a conformational epitope on the Heme-binding domain of Flb2 (residues 1-99). Specifically, the epitope consists of Asn30, residues 63 to 65 (GluPro-Leu) and 67 to 70 (Ala-Pro-Asn-Val), Asp72, Tyr74, and the two heme propionate groups. |
| Application Notes: | ELISA indicated that the antibody, either the IgG or the Fab fragment, was able to recognize the heme-binding domain of flavocytochrome b2. (PMID: 8674650) For the Fab fragment, Kd values of 80 nM and 30 nM were determined in ELISA competition experiments and fluorescence quenching titrations, respectively. (PMID: 9521665) |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |