Mfg Part Number
Ab02951-1.1
Vector Labs Ab02951-1.1 Anti-HCV IRES junction IIIabc [HCV2], Mouse IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by panning against HCV IRES construct.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | HCV2 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; Therapeutic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody recognizes a region located around the four-way junction structure – JIIIabc. In particular, the antibody interacts with the four unpaired nucleotides (A162, G163, U164 and A165) from the IIIa loop and an unpaired nucleotide (G233) and three base-paired nucleotides (G231, C232 and C237) from IIIc. |
| Application Notes: | Specificity of this antibody was confirmed by ELISA tests. The antibody binds the RNA with dissociation constants of 32 ± 7 nM specifically recognizing the so-called junction IIIabc (JIIIabc). In vitro translation assay demonstrated that the antibody binds to junction IIIabc of HCV IRES inhibiting IRES-mediated translation in a concentration-dependent manner, which indicates this antibody might be used in the development of the therapeutic agents (PMID: 31765566) |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |