Mfg Part Number
Ab02953-23.0
Vector Labs Ab02953-23.0 Anti-Integrin alpha V [17E6], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with of purified placental αvβ3.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 17E6 |
| Synonyms: | 272-17E6; Vitronectin receptor; Vitronectin receptor subunit alpha; CD antigen: CD51; Integrin alpha-V heavy chain; Integrin alpha-V light chain; |
| Amount: | 200 μg |
| Application Codes Clone: | FACS; immunoprecipitation; ELISA; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody reacts with extracellular domains on human αv-integrin chains. |
| Application Notes: | αv-Integrin is an integral membrane protein involved in cell adhesion. Antibody screening by ELISA revealed the antibody reacted specifically with αvβ3. In ELISA on fixed cells (‘CELISA’) the antibody showed a reaction pattern consistent with its recognition of the αv integrin chain (cells expressing αvβ3 and αvβ5 (M21)). The ability of the antibody to recognize native αv integrins was tested by FACS analysis, where the αv-expressing cells (M21) reacted strongly with the antibody. Immunoprecipitation from surface labelled cells showed the antibody detected αv integrin in M21 cells. Furthermore, αv-integrins can function as receptors for vitronectin. However, the antibody had the ability to disrupt stable interaction between vitronectin and αvβ3, and blocked the growth of M21 tumors in nude mice. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |