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Mfg Part Number

Ab02956-23.0

Vector Labs Ab02956-23.0 Anti-HIV capsid protein p24 [13B5], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing mice with recombinant HIV-1 capsid protein p24.

$517.40

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Description

Product Class: Purified
Clone ID: 13B5
Synonyms: P24; CA
Amount: 200 μg
Application Codes Clone: ELISA; SPR
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody specifically recognizes an epitope on the C-terminal domain of HIV capsid protein p24. In particular, the Fab recognizes a region in the C-terminal domain of p24 in the loop between helices H10 and H11 (residues 205–213, centered around Pro207).
Application Notes: p24 is the major capsid protein of HIV and plays an active structural role both as part of the Gag polyprotein and in its processed form during virus assembly, maturation and disassembly. The association and dissociation rates and affinity constant for the formation of the 13B5 Fab complex with RH24 (the recombinant HIV-1 capsid protein) have been determined by surface plasmon resonance measurements (with affinity of 29nM). (PMID:11080628) PLA particles with surface adsorbed antigens p24 and gp120 were studied as new vaccine carriers. Competition ELISA was performed to investigate the influence of PLA adsorption on integrity and accessibility at the epitope level. Nanoparticle-adsorbed protein p24 was recognized by the antibody, indicating that the antigenicity and the structural integrity of PLA-adsorbed antigens was extremely well conserved. (PMID: 16919350)

Product Class:	Purified
Clone ID:	13B5
Synonyms:	P24; CA
Amount:	200 μg
Application Codes Clone:	ELISA; SPR
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody specifically recognizes an epitope on the C-terminal domain of HIV capsid protein p24. In particular, the Fab recognizes a region in the C-terminal domain of p24 in the loop between helices H10 and H11 (residues 205–213, centered around Pro207).
Application Notes:	p24 is the major capsid protein of HIV and plays an active structural role both as part of the Gag polyprotein and in its processed form during virus assembly, maturation and disassembly. The association and dissociation rates and affinity constant for the formation of the 13B5 Fab complex with RH24 (the recombinant HIV-1 capsid protein) have been determined by surface plasmon resonance measurements (with affinity of 29nM). (PMID:11080628) PLA particles with surface adsorbed antigens p24 and gp120 were studied as new vaccine carriers. Competition ELISA was performed to investigate the influence of PLA adsorption on integrity and accessibility at the epitope level. Nanoparticle-adsorbed protein p24 was recognized by the antibody, indicating that the antigenicity and the structural integrity of PLA-adsorbed antigens was extremely well conserved. (PMID: 16919350)

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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