Mfg Part Number
Ab02965-23.0
Vector Labs Ab02965-23.0 Anti-CD95 [LOB3-11], Rabbit IgG, kappa (200 μg)
Quick Overview
The mouse version of this antibody was raised by immunizing a mouse with a fusion protein comprising extracellular domain of human Fas linked to human Fc.
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| Product Class: | Purified |
|---|---|
| Clone ID: | LOB3-11 |
| Synonyms: | Tumor necrosis factor receptor superfamily member 6; FAS; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IF; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for human CD95. CD95 is a protein that has an important role in the apoptosis of cells. Due to this property is can play a role in cancer. |
| Application Notes: | To determine the involvement of caspases in apoptosis induced by CD20, a western blot was performed using the mouse version on this antibody on Daudi and Raji cells treated with anti-CD20. Furthermore, the effect of this antibody on mitochondrial permeability was determined. An increase in permeability was indeed observed after treatment with this antibody (Chan et al, 2003; pmid:14500384). Cytospin preparations from MOLT-4 and Jurkat T-cells were stained for immunofluorescence using the mouse version of this antibody to study whether gal-1 interferes with the Fas-associated apoptosis cascade in the T-cell line (Brandt et al, 2008; pmid:18288482). To study whether the expression of the proapoptotic Fas/FasL in the implantation site in impaired during abortion, placental tissue from woman with either voluntary or involuntary abortion was stained for immunohistochemistry using the mouse version of this antibody (Minas et al, 2007; pmid:17704118). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |