Vector Labs Ab02967-10.3 Anti-CD79b [AT107-2], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: AT107-2
Heavy Chain Modification: Fc Silent™
Synonyms: B-cell antigen receptor complex-associated protein beta chain; CD79B; B-cell-specific glycoprotein B29; Ig-beta; Immunoglobulin-associated B29 protein
Amount: 200 μg
Application Codes Clone: FC; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody specific for the intracellular domain of human CD79b. CD79b is required in tandem with CD79A for initiation of the signal transduction cascade induced by the B-cell antigen receptor complex which leads to internalization of the complex, trafficking to late endosomes and antigen presentation.
Application Notes: To study chronic lymphocytic leukemia, immunohistochemistry was performed on tissues from patients with CLL or RT using the rat version of this antibody (Allan et al, 2019). Tissue from non-Hodgkin lymphoma and chronic lymphocytic leukemia patients was stained for immunohistochemistry using the rat version of this antibody to create an antibody–drug conjugate consisting of a humanized anti-CD79b antibody that is conjugated to monomethylauristatin E. This construct is supposed to help the patients with these forms of cancer (Dornan et al, 2009; pmid:19633198). To investigate anti CD79b clone DCDS0780A as a possible therapeutic agent against B-NHL, immunohistochemistry was performed on tissues from B-NHL patients that had relapsed after or failed to respond to at least one prior treatment regimen using the rat version of this antibody (Herrera et al, 2022; pmid:34980599). To determine the expression of CD79b in tumor cells immunohistochemistry was performed on tissues from R/R DLBCL patients using the rat version of this antibody (Sehn et al, 2019; pmid:31693429). To determine the lymphoma isotype of dogs, flow cytometry was performed on lymph node cells using the rat version of this antibody (Thalheim et al, 2013; pmid:24112291). To understand immune activity in koalas, flow cytometry was performed on peripheral blood leukocytes of koalas using the rat version of this antibody (Lau et al, 2012; pmid:22551982). To characterize a CD4+ CD25 high FOXP3 high regulatory T-cell population in dogs, flow cytometry was used on PMBCs using the rat version of this antibody (Pinheiro et al, 2011; pmid:20880379).

Product Class:	Purified
Clone ID:	AT107-2
Heavy Chain Modification:	Fc Silent™
Synonyms:	B-cell antigen receptor complex-associated protein beta chain; CD79B; B-cell-specific glycoprotein B29; Ig-beta; Immunoglobulin-associated B29 protein
Amount:	200 μg
Application Codes Clone:	FC; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody specific for the intracellular domain of human CD79b. CD79b is required in tandem with CD79A for initiation of the signal transduction cascade induced by the B-cell antigen receptor complex which leads to internalization of the complex, trafficking to late endosomes and antigen presentation.
Application Notes:	To study chronic lymphocytic leukemia, immunohistochemistry was performed on tissues from patients with CLL or RT using the rat version of this antibody (Allan et al, 2019). Tissue from non-Hodgkin lymphoma and chronic lymphocytic leukemia patients was stained for immunohistochemistry using the rat version of this antibody to create an antibody–drug conjugate consisting of a humanized anti-CD79b antibody that is conjugated to monomethylauristatin E. This construct is supposed to help the patients with these forms of cancer (Dornan et al, 2009; pmid:19633198). To investigate anti CD79b clone DCDS0780A as a possible therapeutic agent against B-NHL, immunohistochemistry was performed on tissues from B-NHL patients that had relapsed after or failed to respond to at least one prior treatment regimen using the rat version of this antibody (Herrera et al, 2022; pmid:34980599). To determine the expression of CD79b in tumor cells immunohistochemistry was performed on tissues from R/R DLBCL patients using the rat version of this antibody (Sehn et al, 2019; pmid:31693429). To determine the lymphoma isotype of dogs, flow cytometry was performed on lymph node cells using the rat version of this antibody (Thalheim et al, 2013; pmid:24112291). To understand immune activity in koalas, flow cytometry was performed on peripheral blood leukocytes of koalas using the rat version of this antibody (Lau et al, 2012; pmid:22551982). To characterize a CD4+ CD25 high FOXP3 high regulatory T-cell population in dogs, flow cytometry was used on PMBCs using the rat version of this antibody (Pinheiro et al, 2011; pmid:20880379).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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