Mfg Part Number
Ab02977-3.0
Vector Labs Ab02977-3.0 Anti-B7-H4 [S2], Mouse IgG2b, lambda (200 μg)
Quick Overview
The original antibody was generated by immunizing female white leghorn chickens with 50 µg of recombinant B7-H4 protein (Phe29 to Ala258) through intramuscular injection. The spleens of the chickens were harvested 7 days after the final immunization to construct an scFv antibody library. The antibody was isolated by panning against B7-H4 protein.
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| Product Class: | Purified |
|---|---|
| Clone ID: | S2 |
| Synonyms: | B7H4; B7 homolog 4; B7h.5; O8E; Immune costimulatory protein B7-H4; B7S1; Protein B7S1; B7x; T-cell costimulatory molecule B7x; VTCN1; V-set domain-containing T-cell activation inhibitor 1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; Block |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 33716253 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length chimeric human antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds a linear epitope comprising amino acids (VIQWLKEGVLGLVHEFK) of the human B7-H4. |
| Application Notes: | This antibody recognized intact wild type human B7-H4 and linear epitope comprising amino acids 'VIQWLKEGVLGLVHEFK' in an ELISA. This antibody was also capable or recognizing B7-H4 in a western blot assay. The binding of this antibody to B7-H4 protein can effectively neutralize the immunosuppression response induced by B7-H4 proteins, thus enabling T-cells to retain their activation status (PMID: 33716253). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |