Vector Labs Ab02983-10.0 Anti-Leu-7 [HNK-1], Human IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: HNK-1
Synonyms: CD57; leu7; N-CAM; HNK-1 carbohydrate epitope; myelin-associated glycoprotein; MAG; oligodendrocyte; NK-1; HNK1
Amount: 200 μg
Application Codes Clone: IF; FC; IHC; immunoperoxidase staining
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody recognizes the Leu7, which is an oligosaccharide antigenic determinant expressed on a peripheral blood subpopulation of ~15% of lymphocytes that comprises almost all the natural killer (NK) and killer (K) cells but not other blood cells like monocytes, granulocytes, erythrocytes, and platelets (PMID: 6790607). This antibody is also capable of recognizing Myelin-associated glycoprotein (MAG) which is a quantitatively minor component in both peripheral and central myelin sheaths that is thought to have a role in cell–cell interactions within the nervous system (PMID: 6196641).
Application Notes: Indirect immunofluorescence was used to determine the reactivity of this antibody with certain human cultured T cell lines (HSB-2 and MOLT-4 but not MOLT-3) but not with other lines of B cell or phagocytic cell origin. This antibody reacted with 15.1 +/- 7.1% of normal blood lymphocytes but was unreactive with monocytes, granulocytes, erythrocytes, and platelets. This antibody was also used to separate HNK-1+ cells using flow cytometry (PMID: 6790607). The original mouse antibody was used in the immunoperioxidase staining of sections of spleen, tonsils, and lymph nodes where it has been specifically located within the germinal centers, while in the thymus few scattered cells were identified as HNK-1 antigen positive (PMID: 6195296). This antibody could also recognize an antigenic determinant (MAG) of human central and peripheral nervous system white matter by immunoperoxidase staining of tissue sections (PMID: 6196641). This antibody was used in the immunohistochemical recognition of human nerve sheath tumors and neuroepithelial tumors (PMID: 2418621; 3515832).

Product Class:	Purified
Clone ID:	HNK-1
Synonyms:	CD57; leu7; N-CAM; HNK-1 carbohydrate epitope; myelin-associated glycoprotein; MAG; oligodendrocyte; NK-1; HNK1
Amount:	200 μg
Application Codes Clone:	IF; FC; IHC; immunoperoxidase staining
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody recognizes the Leu7, which is an oligosaccharide antigenic determinant expressed on a peripheral blood subpopulation of ~15% of lymphocytes that comprises almost all the natural killer (NK) and killer (K) cells but not other blood cells like monocytes, granulocytes, erythrocytes, and platelets (PMID: 6790607). This antibody is also capable of recognizing Myelin-associated glycoprotein (MAG) which is a quantitatively minor component in both peripheral and central myelin sheaths that is thought to have a role in cell–cell interactions within the nervous system (PMID: 6196641).
Application Notes:	Indirect immunofluorescence was used to determine the reactivity of this antibody with certain human cultured T cell lines (HSB-2 and MOLT-4 but not MOLT-3) but not with other lines of B cell or phagocytic cell origin. This antibody reacted with 15.1 +/- 7.1% of normal blood lymphocytes but was unreactive with monocytes, granulocytes, erythrocytes, and platelets. This antibody was also used to separate HNK-1+ cells using flow cytometry (PMID: 6790607). The original mouse antibody was used in the immunoperioxidase staining of sections of spleen, tonsils, and lymph nodes where it has been specifically located within the germinal centers, while in the thymus few scattered cells were identified as HNK-1 antigen positive (PMID: 6195296). This antibody could also recognize an antigenic determinant (MAG) of human central and peripheral nervous system white matter by immunoperoxidase staining of tissue sections (PMID: 6196641). This antibody was used in the immunohistochemical recognition of human nerve sheath tumors and neuroepithelial tumors (PMID: 2418621; 3515832).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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