Vector Labs Ab02985-8.1 Anti-Sulfatide [O4], Rat IgG2b, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: O4
Synonyms: Oligodendrocyte; sulfated galactolipids; 3’-sulfogalactosylceramide; ; 3’-sulfogalactosyl 1-0-alkyl ether 2-acylglycerol; seminolipid; cholesterol; 04
Amount: 200 μg
Application Codes Clone: IF; ELISA; dot-blot; ICC; IHC; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgM format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody reacts with sulfatide and other sulphated glycolipids found on the surface of early oligodendrocyte progenitors. This antibody also reacted with seminolipid and cholesterol (PMID: 2600978). Oligodendrocytes produce myelin sheath to cover the axons in the central nervous system to support rapid nerve conduction.
Application Notes: Indirect immunofluorescence in cultures of early postnatal mouse cerebellum, cerebrum, spinal cord, optic nerve, and retina was used to study the localization of the antigen bound by this antibody. The original IgM version of this antibody has been used in complement-dependent cytotoxic elimination of cerebellar oligodendrocytes in culture (PMID: 6786942). The reactivity of this antibody towards sulphated glycolipids like sulfatide, seminolipid and cholesterol was texted using ELISA and dot-blots (PMID: 2600978). This antibody along with another oligodendrocyte antibody O4 and oligodendroglial precursor cells (OPC) antibody A2B5 can be used for the immunocytochemical study the developmental stages of oligodendrocytes (PMID: 28230075). O4 antibody specific antigens are expressed on early dendrocyte progenitor and O1 antigens are expressed on late oligodendrocyte progenitors (PMID: 6178060). This antibody was used in the flow cytometric characterization of mouse oligodendroglial cell populations for study of mouse demyelinating disease (PMID: 25247590).

Product Class:	Purified
Clone ID:	O4
Synonyms:	Oligodendrocyte; sulfated galactolipids; 3’-sulfogalactosylceramide; ; 3’-sulfogalactosyl 1-0-alkyl ether 2-acylglycerol; seminolipid; cholesterol; 04
Amount:	200 μg
Application Codes Clone:	IF; ELISA; dot-blot; ICC; IHC; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgM format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody reacts with sulfatide and other sulphated glycolipids found on the surface of early oligodendrocyte progenitors. This antibody also reacted with seminolipid and cholesterol (PMID: 2600978). Oligodendrocytes produce myelin sheath to cover the axons in the central nervous system to support rapid nerve conduction.
Application Notes:	Indirect immunofluorescence in cultures of early postnatal mouse cerebellum, cerebrum, spinal cord, optic nerve, and retina was used to study the localization of the antigen bound by this antibody. The original IgM version of this antibody has been used in complement-dependent cytotoxic elimination of cerebellar oligodendrocytes in culture (PMID: 6786942). The reactivity of this antibody towards sulphated glycolipids like sulfatide, seminolipid and cholesterol was texted using ELISA and dot-blots (PMID: 2600978). This antibody along with another oligodendrocyte antibody O4 and oligodendroglial precursor cells (OPC) antibody A2B5 can be used for the immunocytochemical study the developmental stages of oligodendrocytes (PMID: 28230075). O4 antibody specific antigens are expressed on early dendrocyte progenitor and O1 antigens are expressed on late oligodendrocyte progenitors (PMID: 6178060). This antibody was used in the flow cytometric characterization of mouse oligodendroglial cell populations for study of mouse demyelinating disease (PMID: 25247590).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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