Mfg Part Number
Ab02988-23.0
Vector Labs Ab02988-23.0 Anti-Cotinine [CT#45], Rabbit IgG, lambda (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with a cotinine–albumin conjugate.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | CT#45 |
| Synonyms: | 486-56-6; ChEBI:68641; (S)-Cotinine; Cotinina; Cotininum |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds cotinine. Cotinine is an alkaloid found in tobacco and is also the predominant metabolite of nicotine. It is used as a biomarker for exposure to tobacco smoke. |
| Application Notes: | This antibody was used in the development of an ELISA for determining urinary cotinine to monitor tobacco smoke exposure. This ELISA produced dose–response curves for cotinine ranging from 0.40–100 ng per assay and was able to discriminate nicotine, cotinine N-oxide, cotinine N-glucuronide (cross-reactivity was each <0.5%, taking cotinine as 100%), (3′R, 5′S)-3′-hydroxycotinine O-glucuronide (0.6%) and (R, S)-norcotinine (1.5%). Another major nicotine metabolite, (3′R, 5′S)-3′-hydroxycotinine, showed 8.4% cross-reactivity with this antibody (Kobayashi, 2011) |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |