Mfg Part Number
Ab02994-2.0
Vector Labs Ab02994-2.0 Anti-Diethoxyphosphotyrosine [DepY], Mouse IgG2a, kappa (200 μg)
Quick Overview
The diethoxyphosphorylated (OP) peptides cross-linked to four different carrier proteins were used to immunize and boost mice. Mice were immunized with BSA conjugated to 5 peptides diethoxyphosphorylated on tyrosine: PVYSR-OP, LYGLPR-OP, KYAOP, HYRGPA-OP, and EPNVSY-OP. Mice were boosted with OVA conjugated to three peptides diethoxyphosphorylated on tyrosine, RSLYAS-OP, YGGFL-OP, and YPF-OP, and HSA conjugated to PPYRM-OP and QYDVRK-OP. Later on the hybridomas were screened using lysozyme conjugated to RARYEM-OP, GGYR-OP, and KYK-OP.
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| Product Class: | Purified |
|---|---|
| Clone ID: | DepY |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; BIAcore; Octet technology |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds diethoxyphosphotyrosine (chlorpyrifos oxon adduct). |
| Application Notes: | This antibody was characterized in order to determine its binding affinity and binding specificity. The original mouse antibody recognized diethoxyphosphotyrosine independent of the amino acid sequence around the modified tyrosine and independent of the identity of the carrier protein or peptide in an ELISA and western blotting assay. The original mouse IgG2a version of this antibody had an IC50 of 3 × 10−9 M in a competition assay with OP tubulin. Kd values measured by BIAcore and OctetRED96 were 10−8 M for OP-peptides and 1 × 10−12 M for OP-proteins. The limit of detection measured on western blots hybridized with 0.14 μg/mL of depY was 0.025 μg of human albumin conjugated to YGGFL-OP (PMID: 29137457). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |