Vector Labs Ab02996-13.12 Anti-Monkeypox and Vaccinia A13 protein [11F7], Human IgG4-S228P, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 11F7
Synonyms: VACV; Mpox; monkeypox virus; Protein P8; A13L; vaccinia virus; smallpox virus; VACV virion membrane proteins; Virion membrane protein A13; VACWR132
Amount: 100 μg
Application Codes Clone: ELISA; WB; IF; SPR; neutralize
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds the A13 membrane protein of the monkeypox virus and vaccinia virus. It recognizes an epitope comprising of a 10-amino acid sequence conserved in all orthopoxviruses, including viriola virus and monkeypox virus.
Application Notes: The original IgG2a version of this antibody bound to recombinant A13 protein expressed in E. coli, MBP conjugated VACV A13 in an ELISA. The original IgG2a antibody bound recombinant A13 proteins from E. coli with an affinity of 3.4 nM as detected by surface plasmon resonance. This antibody was also capable of recognizing a 12-kda protein in a Western blot of VACV-infected HeLa cells. This antibody stained viral factories and virion-size particles in immunofluorescence analysis of infected cells. This antibody was also reported to neutralized VACV mature virions in a plaque reduction assay. Passive immunization of mice with this antibody protected mice against intranasal VACV infection. This antibody generated against smallpox vaccine might contribute to immune protection against orthopoxviruses (PMID: 21810533). In Q3 of 2024, the WHO declared monkeypox a Public Health Emergency of International Concern. This product serves as a powerful tool for related research efforts.

Product Class:	Purified
Clone ID:	11F7
Synonyms:	VACV; Mpox; monkeypox virus; Protein P8; A13L; vaccinia virus; smallpox virus; VACV virion membrane proteins; Virion membrane protein A13; VACWR132
Amount:	100 μg
Application Codes Clone:	ELISA; WB; IF; SPR; neutralize
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds the A13 membrane protein of the monkeypox virus and vaccinia virus. It recognizes an epitope comprising of a 10-amino acid sequence conserved in all orthopoxviruses, including viriola virus and monkeypox virus.
Application Notes:	The original IgG2a version of this antibody bound to recombinant A13 protein expressed in E. coli, MBP conjugated VACV A13 in an ELISA. The original IgG2a antibody bound recombinant A13 proteins from E. coli with an affinity of 3.4 nM as detected by surface plasmon resonance. This antibody was also capable of recognizing a 12-kda protein in a Western blot of VACV-infected HeLa cells. This antibody stained viral factories and virion-size particles in immunofluorescence analysis of infected cells. This antibody was also reported to neutralized VACV mature virions in a plaque reduction assay. Passive immunization of mice with this antibody protected mice against intranasal VACV infection. This antibody generated against smallpox vaccine might contribute to immune protection against orthopoxviruses (PMID: 21810533). In Q3 of 2024, the WHO declared monkeypox a Public Health Emergency of International Concern. This product serves as a powerful tool for related research efforts.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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