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Mfg Part Number

Ab02997-23.0

Vector Labs Ab02997-23.0 Anti-PorA protein [207,B-4], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing mice with outer membrane vesicles from Neisseria meningitidis strain 188/87 (B:15:P1.7,16d ET-5).

$517.40

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Description

Product Class: Purified
Clone ID: 207,B-4
Synonyms: PIA; Protein IA; Class 1 protein; P1.7; P1.7 serosubtype; Major outer membrane protein P.IA
Amount: 200 μg
Application Codes Clone: ELISA; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody recognizes the P1.7 loop of the PorA protein of Neisseria meningitidis. Epitope mapping revealed that this antibody specifically binds a sequence 'ASGQ'. The binding of this antibody was enhanced by the addition of a C-terminal valine residue (ASGQv).
Application Notes: This antibody was reported to have bactericidal and opsonophagocytic effects for P1.7-expressing meningococci. This antibody demonstrated high opsonophagocytic activity with >80% of polymorphonuclear leukocytes (PMNs) exhibiting measurable respiratory burst (RB) at a concentration of the MAbs of <10 μg/ml. The phagocytic activity was tested against log-phase-growth strain 44/76-SL bacteria by using human serum as a complement source, human PMNs as effector cells, and increasing concentrations of the antibody as the opsonin. The test was performed by using flow cytometry to measure the respiratory burst induced in the effector cells following phagocytosis of the test strain. The epitope mapping of this antibody was done using ELISA (PMID: 10722576).

Product Class:	Purified
Clone ID:	207,B-4
Synonyms:	PIA; Protein IA; Class 1 protein; P1.7; P1.7 serosubtype; Major outer membrane protein P.IA
Amount:	200 μg
Application Codes Clone:	ELISA; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody recognizes the P1.7 loop of the PorA protein of Neisseria meningitidis. Epitope mapping revealed that this antibody specifically binds a sequence 'ASGQ'. The binding of this antibody was enhanced by the addition of a C-terminal valine residue (ASGQv).
Application Notes:	This antibody was reported to have bactericidal and opsonophagocytic effects for P1.7-expressing meningococci. This antibody demonstrated high opsonophagocytic activity with >80% of polymorphonuclear leukocytes (PMNs) exhibiting measurable respiratory burst (RB) at a concentration of the MAbs of <10 μg/ml. The phagocytic activity was tested against log-phase-growth strain 44/76-SL bacteria by using human serum as a complement source, human PMNs as effector cells, and increasing concentrations of the antibody as the opsonin. The test was performed by using flow cytometry to measure the respiratory burst induced in the effector cells following phagocytosis of the test strain. The epitope mapping of this antibody was done using ELISA (PMID: 10722576).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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