Vector Labs Ab03001-10.3 Anti-gHgL [CL40], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: CL40
Heavy Chain Modification: Fc Silent™
Synonyms: Envelope glycoprotein H; Envelope glycoprotein L
Amount: 200 μg
Application Codes Clone: FC; neutralizing; WB; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody binds to an epitope in gH D-II that overlaps substantially with a region observed to interact with the gp42 HP. In particular the antibody interacts with gH D-II using light chain contacts composed of L-CDR3 with the N-terminal end of gH:2α-7 (helix 7 in gH:D-II), and L-CDR1 with the loop connecting gH:2α-4 and gH:2α-5. Key residue interactions include hydrogen bonds between gH:D284 and L-CDR3:Y94, gH:E286 and L-CDR3:N92, and the backbone of gH:V243 and L-CDR1:N32. There is also minimal lateral contact with gH D-III between L-CDR1: Q27 and the C-terminal end of gH:3α-6, and VL:D1 with gH:N508 on the other side. The antibody heavy chain contacts with gH include H-CDR2:D52 and H-CDR2:D55, forming salt bridges with gH:R184, and backbone H-bonds between H-CDR3: L100 and H-CDR3:L101 with gH:H239.
Application Notes: The antibody blocks EBV-mediated membrane fusion with both B cells and epithelial cells and it retains its inhibitory activity as a monomeric Fab. Flow cytometry cell counts showed that Fab fragment inhibits virus entry. Neutralization experiments with epithelial cells using an EBV-GFP reporter virus and purified Fab were performed. Fluorescence images obtained at 24 h postinfection and flow cytometry cell counts showed that the Fab inhibits virus entry. Fab inhibition of cell–cell fusion and EBV infection exhibited similar concentration dependence. (PMID: 28939750) The cell-surface expression of gHgL was evaluated using the antibody by CELISA. (PMID: 27929061)

Product Class:	Purified
Clone ID:	CL40
Heavy Chain Modification:	Fc Silent™
Synonyms:	Envelope glycoprotein H; Envelope glycoprotein L
Amount:	200 μg
Application Codes Clone:	FC; neutralizing; WB; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody binds to an epitope in gH D-II that overlaps substantially with a region observed to interact with the gp42 HP. In particular the antibody interacts with gH D-II using light chain contacts composed of L-CDR3 with the N-terminal end of gH:2α-7 (helix 7 in gH:D-II), and L-CDR1 with the loop connecting gH:2α-4 and gH:2α-5. Key residue interactions include hydrogen bonds between gH:D284 and L-CDR3:Y94, gH:E286 and L-CDR3:N92, and the backbone of gH:V243 and L-CDR1:N32. There is also minimal lateral contact with gH D-III between L-CDR1: Q27 and the C-terminal end of gH:3α-6, and VL:D1 with gH:N508 on the other side. The antibody heavy chain contacts with gH include H-CDR2:D52 and H-CDR2:D55, forming salt bridges with gH:R184, and backbone H-bonds between H-CDR3: L100 and H-CDR3:L101 with gH:H239.
Application Notes:	The antibody blocks EBV-mediated membrane fusion with both B cells and epithelial cells and it retains its inhibitory activity as a monomeric Fab. Flow cytometry cell counts showed that Fab fragment inhibits virus entry. Neutralization experiments with epithelial cells using an EBV-GFP reporter virus and purified Fab were performed. Fluorescence images obtained at 24 h postinfection and flow cytometry cell counts showed that the Fab inhibits virus entry. Fab inhibition of cell–cell fusion and EBV infection exhibited similar concentration dependence. (PMID: 28939750) The cell-surface expression of gHgL was evaluated using the antibody by CELISA. (PMID: 27929061)

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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