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Mfg Part Number

Ab03002-10.3

Vector Labs Ab03002-10.3 Anti-gHgL [CL59], Human IgG1, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Vector Labs Ab03002-10.3 Anti-gHgL [CL59], Human IgG1, Fc Silent™, kappa (200 μg)

$517.40

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Description

Product Class: Purified
Clone ID: CL59
Heavy Chain Modification: Fc Silent™
Synonyms: Envelope glycoprotein H; Envelope glycoprotein L
Amount: 200 μg
Application Codes Clone: FC; neutralizing; WB; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody binds an epitope in gH D-IV, proximal to the viral membrane and distant from known receptor binding sites. In particular, the antibody binds to the back side of gH D-III/D-IV close to gH D-III residues 494–503 (loop between 3α-8 and 3α-9), 456–468 (3α-6 helix), and 406–415 (loop between 3α-3 and 3α-4), and gH D-IV loop residues 645–656 (loop between 4α-1 and 4β-9), 623–626 (loop between 4β-7 and 4β-8), and 568–577 (loop between 4β-3 and 4β-4).
Application Notes: The antibody blocks EBV-mediated membrane fusion with both B cells and epithelial cells. Flow cytometry cell counts showed that Fab fragment inhibits virus entry. Neutralization experiments with epithelial cells using an EBV-GFP reporter virus and purified Fab. Fluorescence images obtained at 24 h postinfection and flow cytometry cell counts showed that the Fab inhibits virus entry. The Fab exhibited ∼50% inhibition of EBV infection at 150 nM, where no inhibition of cell–cell membrane fusion was observed. (PMID: 28939750) The cell-surface expression of gHgL was evaluated using the antibody by CELISA. (PMID: 27929061)

Product Class:	Purified
Clone ID:	CL59
Heavy Chain Modification:	Fc Silent™
Synonyms:	Envelope glycoprotein H; Envelope glycoprotein L
Amount:	200 μg
Application Codes Clone:	FC; neutralizing; WB; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody binds an epitope in gH D-IV, proximal to the viral membrane and distant from known receptor binding sites. In particular, the antibody binds to the back side of gH D-III/D-IV close to gH D-III residues 494–503 (loop between 3α-8 and 3α-9), 456–468 (3α-6 helix), and 406–415 (loop between 3α-3 and 3α-4), and gH D-IV loop residues 645–656 (loop between 4α-1 and 4β-9), 623–626 (loop between 4β-7 and 4β-8), and 568–577 (loop between 4β-3 and 4β-4).
Application Notes:	The antibody blocks EBV-mediated membrane fusion with both B cells and epithelial cells. Flow cytometry cell counts showed that Fab fragment inhibits virus entry. Neutralization experiments with epithelial cells using an EBV-GFP reporter virus and purified Fab. Fluorescence images obtained at 24 h postinfection and flow cytometry cell counts showed that the Fab inhibits virus entry. The Fab exhibited ∼50% inhibition of EBV infection at 150 nM, where no inhibition of cell–cell membrane fusion was observed. (PMID: 28939750) The cell-surface expression of gHgL was evaluated using the antibody by CELISA. (PMID: 27929061)

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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