Mfg Part Number
Ab03003-10.27
Vector Labs Ab03003-10.27 Anti-FLT3 [EB10], Human Fab fragment, kappa (200 μg)
Quick Overview
The original Fab antibody was isolated from a human Fab phage display by selection of a high affinity antibody binding to the human FLT3.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | EB10 |
| Synonyms: | FLK-2; FLT-3; STK-1; CD135; Fetal liver kinase 2; FL cytokine receptor; stem cell tyosine kinase 1; Fms-like tyrosine kinase 3; Receptor-type tyrosine-protein kinase FLT3 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted human antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody binds an epitope within domain D4 of human FLT3. |
| Application Notes: | The clone was shown to selectively bind to human FLT3 with high affinity, block FL binding and mediated potent anti-tumor activity in xenograft model by a mechanism involving activation of immune effector function. The ability of the antibody to bind the receptor was examined by ELISA (EC50 within the range 0.5-1 nM). The ability of the antibody to block receptor-ligand binding was examined in a FL binding competition ELISA, the antibody blocked the binding of FL to FLT3 with a IC50 within the range of 0.5-1 nM. The clone binds FLT3 with an affinity of 0.158 nM as determined by surface plasmon resonance (SPR). Flow cytometric analysis showed that the antibody bound to the wild-type FLT-3 expressed on EOL-1 cells and also to ITD-mutant FLT3 expressed on BaF3-ITD cells (US8071099B2). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |