Mfg Part Number
Ab03005-3.3
Vector Labs Ab03005-3.3 Anti-FLT3 [D3-D4 (D4-3)], Mouse IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
Vector Labs Ab03005-3.3 Anti-FLT3 [D3-D4 (D4-3)], Mouse IgG2b, Fc Silent™, kappa (200 μg)
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | D3-D4 (D4-3) |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | FLK-2; FLT-3; STK-1; CD135; Fetal liver kinase 2; FL cytokine receptor; stem cell tyosine kinase 1; Fms-like tyrosine kinase 3; Receptor-type tyrosine-protein kinase FLT3 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody binds an epitope within domain D4 of human FLT3. |
| Application Notes: | The clone was shown to selectively bind to human FLT3 with high affinity and to block FL binding. The ability of the antibody to bind the receptor was examined by ELISA (EC50= 0.2 nM). The ability of the antibody to block receptor-ligand binding was examined in a FL binding competition ELISA, the antibody blocked the binding of FL to FLT3 with a IC50 within the range of 7 nM. The clone binds FLT3 with an affinity of 0.27 nM as determined by surface plasmon resonance (SPR). The in vivo efficacy of the clone was determined in SEM-K2 cells, a human ALL cell line expressing wild type FLT3. In comparison to human IgG, the clone treatment all significantly prolonged survival in the SEM-K2 leukemia model (US8071099B2). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |