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Mfg Part Number

Ab03006-1.27

Vector Labs Ab03006-1.27 Anti-Botulinum neurotoxin [BotFab 5], Mouse Fab fragment, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by panning the library against botulinum type B complex.

$517.40

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Description

Product Class: Purified
Clone ID: BotFab 5
Synonyms: BOTOX; BoNT/A; Bontoxilysin-A; Botulinum neurotoxin type A1; Botulinum neurotoxin type A; BoNT/B; Bontoxilysin-B; Botulinum neurotoxin type B; botulinum types A and B toxin complexes.
Amount: 200 μg
Application Codes Clone: ELISA; SPR; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody is specific for the hemagglutinin portion of the botulinum neurotoxin A and B complexes.
Application Notes: The Fab was shown by ELISA to be reactive to botulinum types A and B toxin complexes and not reactive to the 150 kDa NT of type A or B that lacked the non-NT proteins found in the complex. (US5932449A) Surface plasmon resonance demonstrated that the Fab displays a slightly tighter equilibrium binding constant towards botulinum toxin B than toxin A. The dissociation rate constants (kd1 and kd2) with botulinum toxin A are 7.1×10^-3 and 2.3×10^-4 s^-1 (ratio 1:2), while with botulinum toxin B the values are 3.7×10^-3 and 5.7×10^-4 s^-1 (ratio 1:3.5). The Fab antibody was used in an indirect flow cytometric immunoassay to detect the presence of pentavalent toxoid A-B-C-D-E and botulinum toxoid A; the antibody detected the presence of botulinum toxoid A. The limit of sensitivity of the Fab fragment for botulinum toxoid is between 5 and 10 ng/ml (PMID: 10945449)

Product Class:	Purified
Clone ID:	BotFab 5
Synonyms:	BOTOX; BoNT/A; Bontoxilysin-A; Botulinum neurotoxin type A1; Botulinum neurotoxin type A; BoNT/B; Bontoxilysin-B; Botulinum neurotoxin type B; botulinum types A and B toxin complexes.
Amount:	200 μg
Application Codes Clone:	ELISA; SPR; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody is specific for the hemagglutinin portion of the botulinum neurotoxin A and B complexes.
Application Notes:	The Fab was shown by ELISA to be reactive to botulinum types A and B toxin complexes and not reactive to the 150 kDa NT of type A or B that lacked the non-NT proteins found in the complex. (US5932449A) Surface plasmon resonance demonstrated that the Fab displays a slightly tighter equilibrium binding constant towards botulinum toxin B than toxin A. The dissociation rate constants (kd1 and kd2) with botulinum toxin A are 7.1×10^-3 and 2.3×10^-4 s^-1 (ratio 1:2), while with botulinum toxin B the values are 3.7×10^-3 and 5.7×10^-4 s^-1 (ratio 1:3.5). The Fab antibody was used in an indirect flow cytometric immunoassay to detect the presence of pentavalent toxoid A-B-C-D-E and botulinum toxoid A; the antibody detected the presence of botulinum toxoid A. The limit of sensitivity of the Fab fragment for botulinum toxoid is between 5 and 10 ng/ml (PMID: 10945449)

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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