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Mfg Part Number

Ab03009-23.0

Vector Labs Ab03009-23.0 Anti-BRAF V600E protein [600E-7], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing mice with double repeat recombinant BRAF V600E protein 'GDFGLATEKSRW' and BRAF V600 protein 'GDFGLATVKSRW' in equal amount of complete Freund’s adjuvant.

$517.40

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Description

Product Class: Purified
Clone ID: 600E-7
Synonyms: RAFB1; BRAF1; p94; BRAF V600E kinase; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; v-Raf murine sarcoma viral oncogene homolog B1
Amount: 200 μg
Application Codes Clone: ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the V600E mutation of the BRAF protein kinase. BRAF V600E mutation can promote the methylation of the thyrotropin receptor (TSHR) gene promoter, resulting in silencing of TSHR or a significant decrease in expression, and feedback increase of thyrotropin (TSH), which promotes the growth of tumor cells. BRAF V600E gene mutation and telomerase reverse transcriptase (TERT) promoter mutation also play an important role in the occurrence of papillary thyroid carcinoma (PTC).
Application Notes: This antibody can be used for the detection of the BRAF V600E mutation. This antibody is useful in the diagnosis and targeted therapy for papillary thyroid carcinoma. The binding characterization of this antibody for BRAF V660E mutation was done using ELISA. The IgG1 version of the antibody binds the BRAF V600E mutation with a dissociation constant of 0.04 nM and IC50 of the IgG1 antibody against A375 cells was 42nM. This antibody was used in the generation of immunomagnetic beads for the detection of BRAF V600E mutation (CN111285935).

Product Class:	Purified
Clone ID:	600E-7
Synonyms:	RAFB1; BRAF1; p94; BRAF V600E kinase; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; v-Raf murine sarcoma viral oncogene homolog B1
Amount:	200 μg
Application Codes Clone:	ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the V600E mutation of the BRAF protein kinase. BRAF V600E mutation can promote the methylation of the thyrotropin receptor (TSHR) gene promoter, resulting in silencing of TSHR or a significant decrease in expression, and feedback increase of thyrotropin (TSH), which promotes the growth of tumor cells. BRAF V600E gene mutation and telomerase reverse transcriptase (TERT) promoter mutation also play an important role in the occurrence of papillary thyroid carcinoma (PTC).
Application Notes:	This antibody can be used for the detection of the BRAF V600E mutation. This antibody is useful in the diagnosis and targeted therapy for papillary thyroid carcinoma. The binding characterization of this antibody for BRAF V660E mutation was done using ELISA. The IgG1 version of the antibody binds the BRAF V600E mutation with a dissociation constant of 0.04 nM and IC50 of the IgG1 antibody against A375 cells was 42nM. This antibody was used in the generation of immunomagnetic beads for the detection of BRAF V600E mutation (CN111285935).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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