Vector Labs Ab03011-2.3 Anti-OspB [H6831], Mouse IgG2a, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: H6831
Heavy Chain Modification: Fc Silent™
Synonyms: Outer surface protein B
Amount: 200 μg
Application Codes Clone: IF; WB; IP; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is directed against the C terminus of outer surface protein B (OspB), a major lipoprotein of the Lyme disease spirochete, Borrelia burgdorferi. This antibody binds an epitope topologically analogous to the LA-2 epitope of OspA and is centered around OspB Lys-253, a residue essential for recognition by H6831.
Application Notes: Indirect immunofluorescence was used to determine the reactivity of this antibody towards 14 strains of Lyme disease spirochetes. This antibody was also used for the immunoprecipitation of Shelter Island LD spirochete isolate (PMID: 6735474). This antibody capable of detecting B. burgdorferi B311 cell lysates in a western blot. (PMID: 6735474; 7513309). This antibody has an unusual property of having bactericidal effect even in the absence of complement or phagocytes. In time-kill studies, more than 99.9% of strain B31 B. burgdorferi cells were killed after 30 min of exposure to H6831 Fab fragments. It was reported that the whole IgG version of this antibody inhibited B311 cells but did not inhibit the growth of Osp-less B313 cells even at 25 mg/ml (PMID: 7890424). ELISA was used to check the bactericidal activity of this antibody towards whole cells of B. burgdorferi strain B311 after incubation with serial dilutions of this antibody (PMID: 7513309). This antibody was also used to identify its usability in a capture ELISA for detecting various isolates of Borrelia burgdorferi (PMID: 8432812).

Product Class:	Purified
Clone ID:	H6831
Heavy Chain Modification:	Fc Silent™
Synonyms:	Outer surface protein B
Amount:	200 μg
Application Codes Clone:	IF; WB; IP; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is directed against the C terminus of outer surface protein B (OspB), a major lipoprotein of the Lyme disease spirochete, Borrelia burgdorferi. This antibody binds an epitope topologically analogous to the LA-2 epitope of OspA and is centered around OspB Lys-253, a residue essential for recognition by H6831.
Application Notes:	Indirect immunofluorescence was used to determine the reactivity of this antibody towards 14 strains of Lyme disease spirochetes. This antibody was also used for the immunoprecipitation of Shelter Island LD spirochete isolate (PMID: 6735474). This antibody capable of detecting B. burgdorferi B311 cell lysates in a western blot. (PMID: 6735474; 7513309). This antibody has an unusual property of having bactericidal effect even in the absence of complement or phagocytes. In time-kill studies, more than 99.9% of strain B31 B. burgdorferi cells were killed after 30 min of exposure to H6831 Fab fragments. It was reported that the whole IgG version of this antibody inhibited B311 cells but did not inhibit the growth of Osp-less B313 cells even at 25 mg/ml (PMID: 7890424). ELISA was used to check the bactericidal activity of this antibody towards whole cells of B. burgdorferi strain B311 after incubation with serial dilutions of this antibody (PMID: 7513309). This antibody was also used to identify its usability in a capture ELISA for detecting various isolates of Borrelia burgdorferi (PMID: 8432812).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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