Mfg Part Number
Ab03021-1.29
Vector Labs Ab03021-1.29 Anti-HA cleavage site [A3], Mouse Fab fragment, His-Tagged, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing mice with the cleavage site (CS) peptide of hemagglutinin protein, followed by antibody library construction and phage display-based selection.
$692.90
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| Product Class: | Purified |
|---|---|
| Clone ID: | A3 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | Hemagglutinin Cleavage Site; CS HA; Avian Influenza Virus; H5N1 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; IF; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody specifically recognizes the sequence (NS)PQRER observed at the cleavage site of the hemagglutinin protein of the H5N1 avian influenza virus. The antibody does not cross-react with the HA protein of H1N1 or H7N7 strain. |
| Application Notes: | The specificity of this antibody (Fab) was confirmed by ELISA. The epitope mapping of this antibody was done using ELISA. The binding affinity of the Fab fragment towards the cleavage site peptide of HA derived from the HPAI H5N1 virus was found to be Kd= 0,80 nM using surface plasmon resonance. The clone was used for detection of the cleavage site peptide on an open sandwich ELISA, reaching the lower limit of detection (LOD) in the range of 1 to 10 ng/ml. This antibody was also used in the immunofluorescent staining of Madin-Darby canine kidney cells infected with HPAI H5N1 (Dong et al, 2013; pmid:23577205). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |