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Mfg Part Number

Ab03025-10.29-BS

Vector Labs Ab03025-10.29-BS Anti-VP35 [H3], Human Fab fragment, His-Tagged, kappa (1 mg)

VECTOR LABS

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Quick Overview

The original antibody was generated by using phage display technology. The library was constructed on a single human Fab framework with variable domains from subgroups VH3 and Vκ1. The synthetic Fab phage library was screened against the recombinant mVP35 IFN inhibitory domain (IID) protein of Marburg virus.

$5,162.30

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Description

Product Class: Purified
Clone ID: H3
Heavy Chain Modification: His-Tagged
Synonyms: Polymerase cofactor VP35; VP35; mVP35; Marburg VP35; Marburg virus protein 35; sFab H3
Amount: 1 mg
Application Codes Clone: ELISA; crystallography; inhibition
Shipping Temperature: Wet Ice
Buffer Composition: PBS only.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody binds the α-helical subdomain of mVP35 IID. Further, the antibody does not cross-react with Ebola virus VP35 protein.
Application Notes: The specificity of this antibody (Fab) was confirmed by ELISA analysis. The antibody was crystallized with the antigen. The dissociation constants (KD) measured using isothermal-titration calorimetry (ITC) revealed that the antibody binds to mVP35 IID with high affinity (KD = 4.90 ± 1 nM) and it exhibited 1:1 stoichiometry. The scFv format of the antibody was employed to test if the antibody binding interferes with mVP35 function in viral replication, scFv expression inhibited the Marburg polymerase complex in a dose-dependent manner but did not inhibit Ebola minigenome activity (Amatya et al, 2019; pmid:31120240).

Product Class:	Purified
Clone ID:	H3
Heavy Chain Modification:	His-Tagged
Synonyms:	Polymerase cofactor VP35; VP35; mVP35; Marburg VP35; Marburg virus protein 35; sFab H3
Amount:	1 mg
Application Codes Clone:	ELISA; crystallography; inhibition
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS only.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted human antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody binds the α-helical subdomain of mVP35 IID. Further, the antibody does not cross-react with Ebola virus VP35 protein.
Application Notes:	The specificity of this antibody (Fab) was confirmed by ELISA analysis. The antibody was crystallized with the antigen. The dissociation constants (KD) measured using isothermal-titration calorimetry (ITC) revealed that the antibody binds to mVP35 IID with high affinity (KD = 4.90 ± 1 nM) and it exhibited 1:1 stoichiometry. The scFv format of the antibody was employed to test if the antibody binding interferes with mVP35 function in viral replication, scFv expression inhibited the Marburg polymerase complex in a dose-dependent manner but did not inhibit Ebola minigenome activity (Amatya et al, 2019; pmid:31120240).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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