Vector Labs Ab03049-10.0 Anti-VWF [NMC-4], Human IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: NMC-4
Synonyms: F8VWF; von Willebrand Factor; von Willebrand antigen 2; von Willebrand antigen II; Factor VIII related antigen; NMC4
Amount: 200 μg
Application Codes Clone: ELISA; IP; inhibit
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The NMC-4 antibody binds the GP1b-α binding site on the A1 domain of human von Willebrand factor (also know as vWF or Factor VIII related antigen). vWF is a plasma glycoprotein (GP) that circulates in blood as a series of multimers, in association with factor VIII procoagulant protein. It is produced by endothelial cells and megakaryocytes and is involved in the process of hemostasis by engaging in adhesion of platelets to the sites of vascular injury. Mutations in this gene lead to Von Willebrand's disease which causes frequent bleeding.
Application Notes: The binding characterization of this antibody was done using ELISA (Shima et al, 1985). This antibody was used in the initial characterization of the three distinct functional sites on the von Willebrand factor molecule (PMID: 2477370). This antibody can be used for the immunoprecipitation of the von Willebrand factor from a sample (PMID: 7679102). A study reported that this antibody was shown to inhibit its binding to GP Ib, completely abolished SIPA (sheer induced platelet aggregation) under high shear force, even in the presence of epinephrine. However, this antibody only partially inhibited SIPA under low shear force (PMID: 1360339). This antibody is also reported to completely inhibit vWF binding to platelet glycoprotein (GP) lb induced by either ristocetin or botrocetin at an IgG concentration of approximately 10 micrograms/mL, and also blocked binding of asialo-vWF to GP lb (PMID: 1984791).

Product Class:	Purified
Clone ID:	NMC-4
Synonyms:	F8VWF; von Willebrand Factor; von Willebrand antigen 2; von Willebrand antigen II; Factor VIII related antigen; NMC4
Amount:	200 μg
Application Codes Clone:	ELISA; IP; inhibit
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The NMC-4 antibody binds the GP1b-α binding site on the A1 domain of human von Willebrand factor (also know as vWF or Factor VIII related antigen). vWF is a plasma glycoprotein (GP) that circulates in blood as a series of multimers, in association with factor VIII procoagulant protein. It is produced by endothelial cells and megakaryocytes and is involved in the process of hemostasis by engaging in adhesion of platelets to the sites of vascular injury. Mutations in this gene lead to Von Willebrand's disease which causes frequent bleeding.
Application Notes:	The binding characterization of this antibody was done using ELISA (Shima et al, 1985). This antibody was used in the initial characterization of the three distinct functional sites on the von Willebrand factor molecule (PMID: 2477370). This antibody can be used for the immunoprecipitation of the von Willebrand factor from a sample (PMID: 7679102). A study reported that this antibody was shown to inhibit its binding to GP Ib, completely abolished SIPA (sheer induced platelet aggregation) under high shear force, even in the presence of epinephrine. However, this antibody only partially inhibited SIPA under low shear force (PMID: 1360339). This antibody is also reported to completely inhibit vWF binding to platelet glycoprotein (GP) lb induced by either ristocetin or botrocetin at an IgG concentration of approximately 10 micrograms/mL, and also blocked binding of asialo-vWF to GP lb (PMID: 1984791).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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