Vector Labs Ab03056-10.0 Anti-Glycoprotein D [HSV8], Human IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: HSV8
Synonyms: gD; Envelope glycoprotein D
Amount: 200 μg
Application Codes Clone: ELISA; IP; WB; neutralizing; IF; inhibition; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody binds to glycoprotein D (epitope located between residues 234 and 275).
Application Notes: Screening of the phage display for significant binding towards HSV-1 and HSV-2 was done using ELISA; The Fab supernates were tested in immunofluorescence studies with virus infected cells. The protein recognized by the Fab was identified via immunoprecipitation. Western blot confirmed specificity Fab for gD. Fab fragment neutralizes infectivity and prevents cell-to-cell transmission of herpes simplex viruses 1 and 2. The Fab fragment neutralized HSV-1 at 0.25 ug/ml (50% reduction) and HSV-2 at 0.05 ug/ml. This Fab also inhibited plaque formation when applied to virus-infected monolayers, completely abolishing HSV-2 plaque development at 25 ug/ml 72 hours postinfection, indicating the ability of the Fab to prevent cell-to-cell spread of virus (Burioni et al, 1994; PMID:8278393). In vivo experiments showed that the antibody (IgG1) reduced the mortality rate in mice when administrated before HSV type 1 infection and it prolonged survival times when administered up to 24 hours postinfection (Sanna et al, 1996; PMID:8553581). The epitope mapping of this antibody was done using competition ELISA. The antibody demonstrated potent activities against cell-associated virus in a syncytium inhibition assay (De Logu et al, 1998; PMID:9774565). IgG1, Fab and F(ab)2 fragments were tested against HSV-2 in mice in vivo and they were approximately equally protective (Zeitlin et al, 1996; PMID:8918548).

Product Class:	Purified
Clone ID:	HSV8
Synonyms:	gD; Envelope glycoprotein D
Amount:	200 μg
Application Codes Clone:	ELISA; IP; WB; neutralizing; IF; inhibition; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted human antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody binds to glycoprotein D (epitope located between residues 234 and 275).
Application Notes:	Screening of the phage display for significant binding towards HSV-1 and HSV-2 was done using ELISA; The Fab supernates were tested in immunofluorescence studies with virus infected cells. The protein recognized by the Fab was identified via immunoprecipitation. Western blot confirmed specificity Fab for gD. Fab fragment neutralizes infectivity and prevents cell-to-cell transmission of herpes simplex viruses 1 and 2. The Fab fragment neutralized HSV-1 at 0.25 ug/ml (50% reduction) and HSV-2 at 0.05 ug/ml. This Fab also inhibited plaque formation when applied to virus-infected monolayers, completely abolishing HSV-2 plaque development at 25 ug/ml 72 hours postinfection, indicating the ability of the Fab to prevent cell-to-cell spread of virus (Burioni et al, 1994; PMID:8278393). In vivo experiments showed that the antibody (IgG1) reduced the mortality rate in mice when administrated before HSV type 1 infection and it prolonged survival times when administered up to 24 hours postinfection (Sanna et al, 1996; PMID:8553581). The epitope mapping of this antibody was done using competition ELISA. The antibody demonstrated potent activities against cell-associated virus in a syncytium inhibition assay (De Logu et al, 1998; PMID:9774565). IgG1, Fab and F(ab)2 fragments were tested against HSV-2 in mice in vivo and they were approximately equally protective (Zeitlin et al, 1996; PMID:8918548).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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