JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

Ab03057-1.1

Vector Labs Ab03057-1.1 Anti-Adeno associated virus [A20], Mouse IgG1, kappa (200 μg)

VECTOR LABS

Be the first to review this product

Quick Overview

The original antibody was generated by immunizing BALB/c mice with a mixture of recombinant AAV capsid proteins (VP1, VP2, and VP3).

$517.40

Order will ship within 1-3 business days

Description

Product Class: Purified
Clone ID: A20
Synonyms: AAV-2 capsid proteins; Capsid protein VP1
Amount: 200 μg
Application Codes Clone: IP; IF; ELISA; Neutralizing
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG3
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG3 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody recognizes a conformational epitope on assembled capsid proteins of AAV-2 but it fails to detect nonassembled or denatured capsid proteins.
Application Notes: The clone was used to investigate the AAV assembly process on the cellular level. The clone was used to immunoprecipitate capsid proteins from extracts of [35S]methionine-labeled HeLa cells infected with AAV-2 and adenovirus type 2. Immunofluorescence was preformed on AAV-2/adenovirus type 2-coinfected HeLa cells using this antibody (Wistuba et al, 1997; pmid:8995658). Epitope sequences on the capsid surface were identified by enzyme-linked immunoabsorbent assay using AAV-2 mutants and AAV serotypes, peptide scan, and peptide competition experiments. The clone neutralizes infection following receptor attachment by binding an epitope formed during AAV-2 capsid assembly. The antibody bound empty and full AAV-2 particles in an ELISA. In a neutralization assay, incubation of rAAV-2-GFP with A20, on HeLa cells prevented transgene expression. The clone reacted also with AAV-3 as shown by ELISA (Wobus et al, 2000; pmid:10982375). The structure for the complex AAV-2 with Fab' fragment was determined by cryo-electron microscopy (McCraw et al, 2012; pmid:22682774).

Product Class:	Purified
Clone ID:	A20
Synonyms:	AAV-2 capsid proteins; Capsid protein VP1
Amount:	200 μg
Application Codes Clone:	IP; IF; ELISA; Neutralizing
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG3
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG3 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody recognizes a conformational epitope on assembled capsid proteins of AAV-2 but it fails to detect nonassembled or denatured capsid proteins.
Application Notes:	The clone was used to investigate the AAV assembly process on the cellular level. The clone was used to immunoprecipitate capsid proteins from extracts of [35S]methionine-labeled HeLa cells infected with AAV-2 and adenovirus type 2. Immunofluorescence was preformed on AAV-2/adenovirus type 2-coinfected HeLa cells using this antibody (Wistuba et al, 1997; pmid:8995658). Epitope sequences on the capsid surface were identified by enzyme-linked immunoabsorbent assay using AAV-2 mutants and AAV serotypes, peptide scan, and peptide competition experiments. The clone neutralizes infection following receptor attachment by binding an epitope formed during AAV-2 capsid assembly. The antibody bound empty and full AAV-2 particles in an ELISA. In a neutralization assay, incubation of rAAV-2-GFP with A20, on HeLa cells prevented transgene expression. The clone reacted also with AAV-3 as shown by ELISA (Wobus et al, 2000; pmid:10982375). The structure for the complex AAV-2 with Fab' fragment was determined by cryo-electron microscopy (McCraw et al, 2012; pmid:22682774).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews