Mfg Part Number
Ab03060-3.0
Vector Labs Ab03060-3.0 Anti-NP [JS08], Mouse IgG2b, lambda (200 μg)
Quick Overview
A combinatorial fragment of antigen-binding (Fab)antibody phage library based on peripheral blood-derived from five coronavirus disease 2019 (COVID-19) infected donors was constructed. The Fab antibody was obtained and identified by panning with purified NP.
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| Product Class: | Purified |
|---|---|
| Clone ID: | JS08 |
| Synonyms: | N; Nucleoprotein; Nucleocapsid protein; NC; Protein N |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody binds the nucleocapsid protein of SARS-CoV-2. |
| Application Notes: | The specificity of the Fab and the IgG1 formats for SARS-CoV-2 NP was confirmed by ELISA analysis. The IgG1 antibody detected SARA-Cov-2 NP by western blot. Surface plasmon resonance assay was performed to evaluate the binding specificity and affinity between antibody and purified NP; the antibody showed a tight binding affinity to immobilized NP. The antibody was used as a detection antibody in sandwich ELISA, where it was used in a matched pair in conjunction with JS16, with the optimal detection limit below 0.78ng/mL. The epitope of the antibody was analyzed by competition ELISA (Zhang et al, 2020; pmid:33281824). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |