Vector Labs Ab03080-23.0 Anti-LDL Receptor [C7], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: C7
Synonyms: LDLR; Low-density lipoprotein receptor; IgG-C7
Amount: 200 μg
Application Codes Clone: FC; IP; WB; inhibition; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody recognizes the ligand binding domain. It binds to the bovine LDL-R, the human LDL-R but it does not cross react with the LDL-Rs of rat, mouse, Chinese hamster, rabbit and dog. The LDL receptor is a cell surface protein that binds LDL, the major cholesterol-transport protein in plasma, and mediates its endocytosis.
Application Notes: The antibody (IgG2b) was employed for the staining of the LDL receptor in normal fibroblast. The antibody competed with LDL for binding to the LDL receptor of intact fibroblast; complete inhibition was achieved at 37 C but not at 4 C (Beisiegel et al., 1981; PMID: 6271765). The antibody detected LDL receptor from human fibroblasts and bovine adrenal cortex by western blot analysis (Beisiegel et al., 1982; PMID: 6290495). The LDL receptor was immunoprecipitated using this antibody (Davis et al., 1987; PMID: 3494949). This antibody was used for detection of LDL receptor expressed on Chang and CHO cells by flow cytometry (Holst et al., 2001; PMID: 11781697) (Ranheim et al., 2006; PMID: 16740646). Immunofluorescence was performed on liver Chang cells using this antibody, showing that the WT protein was localised on the cell surface whereas the two mutant proteins were localised intracellularly (Holst et al., 2001; PMID: 11781697). The antibody was used to inhibit the binding of LDL to its receptor LDLR in brain capillary endothelial cells. Coincubations of LDL with increasing concentrations of the antibody decreased the rate of passage of the LDL through the monolayer; LDL transcytosis was totally abolished at the concentration of 1 mg/ml of antibody. This confirmed that the LDL receptor is involved receptor-mediated transcytosis (Dehouck et al., 1997; PMID: 9265653).

Product Class:	Purified
Clone ID:	C7
Synonyms:	LDLR; Low-density lipoprotein receptor; IgG-C7
Amount:	200 μg
Application Codes Clone:	FC; IP; WB; inhibition; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody recognizes the ligand binding domain. It binds to the bovine LDL-R, the human LDL-R but it does not cross react with the LDL-Rs of rat, mouse, Chinese hamster, rabbit and dog. The LDL receptor is a cell surface protein that binds LDL, the major cholesterol-transport protein in plasma, and mediates its endocytosis.
Application Notes:	The antibody (IgG2b) was employed for the staining of the LDL receptor in normal fibroblast. The antibody competed with LDL for binding to the LDL receptor of intact fibroblast; complete inhibition was achieved at 37 C but not at 4 C (Beisiegel et al., 1981; PMID: 6271765). The antibody detected LDL receptor from human fibroblasts and bovine adrenal cortex by western blot analysis (Beisiegel et al., 1982; PMID: 6290495). The LDL receptor was immunoprecipitated using this antibody (Davis et al., 1987; PMID: 3494949). This antibody was used for detection of LDL receptor expressed on Chang and CHO cells by flow cytometry (Holst et al., 2001; PMID: 11781697) (Ranheim et al., 2006; PMID: 16740646). Immunofluorescence was performed on liver Chang cells using this antibody, showing that the WT protein was localised on the cell surface whereas the two mutant proteins were localised intracellularly (Holst et al., 2001; PMID: 11781697). The antibody was used to inhibit the binding of LDL to its receptor LDLR in brain capillary endothelial cells. Coincubations of LDL with increasing concentrations of the antibody decreased the rate of passage of the LDL through the monolayer; LDL transcytosis was totally abolished at the concentration of 1 mg/ml of antibody. This confirmed that the LDL receptor is involved receptor-mediated transcytosis (Dehouck et al., 1997; PMID: 9265653).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews