Mfg Part Number
Ab03081-1.1
Vector Labs Ab03081-1.1 Anti-Arginase 1 [mAb5], Mouse IgG1, kappa (200 μg)
Quick Overview
The antibody was generated by immunizing mice with recombinant arginase 1.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | mAb5 |
| Synonyms: | ARG1; Arginase-1; Liver-type arginase; Type I arginase |
| Amount: | 200 μg |
| Application Codes Clone: | Cryo-electron microscopy; Inhibition |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody binds arginase 1. The binding of the antibody does not sterically block the Arginase 1 active site, but it interacts with an amino acid (Arg21) important to substrate binding by arginase 1, thereby inhibiting enzyme activity. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates T-cell-mediated immune response. |
| Application Notes: | The structure and epitope mapping of the complex arginase:antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of mouse arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki of the antibody against mouse Arg1 was 25 ± 1.7 nM (Palte et al., 2021; PMID: 34326456). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |