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Mfg Part Number

Ab03081-23.0

Vector Labs Ab03081-23.0 Anti-Arginase 1 [mAb5], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

The antibody was generated by immunizing mice with recombinant arginase 1.

$517.40

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Description

Product Class: Purified
Clone ID: mAb5
Synonyms: ARG1; Arginase-1; Liver-type arginase; Type I arginase
Amount: 200 μg
Application Codes Clone: Cryo-electron microscopy; Inhibition
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody binds arginase 1. The binding of the antibody does not sterically block the Arginase 1 active site, but it interacts with an amino acid (Arg21) important to substrate binding by arginase 1, thereby inhibiting enzyme activity. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates T-cell-mediated immune response.
Application Notes: The structure and epitope mapping of the complex arginase:antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of mouse arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki of the antibody against mouse Arg1 was 25 ± 1.7 nM (Palte et al., 2021; PMID: 34326456).

Product Class:	Purified
Clone ID:	mAb5
Synonyms:	ARG1; Arginase-1; Liver-type arginase; Type I arginase
Amount:	200 μg
Application Codes Clone:	Cryo-electron microscopy; Inhibition
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody binds arginase 1. The binding of the antibody does not sterically block the Arginase 1 active site, but it interacts with an amino acid (Arg21) important to substrate binding by arginase 1, thereby inhibiting enzyme activity. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates T-cell-mediated immune response.
Application Notes:	The structure and epitope mapping of the complex arginase:antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of mouse arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki of the antibody against mouse Arg1 was 25 ± 1.7 nM (Palte et al., 2021; PMID: 34326456).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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