Mfg Part Number
Ab03083-23.0
Vector Labs Ab03083-23.0 Anti-Butyrylcholinesterase [C191], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated by injecting mice with denatured, recombinant human butyrylcholinesterase expressed in Chinese hamster ovary cells. The recombinant butyrylcholinesterase had been denatured and reduced by boiling in 1% sodium dodecyl sulfate containing 5% mercaptoethanol before it was injected into mice.
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| Product Class: | Purified |
|---|---|
| Clone ID: | C191 |
| Synonyms: | Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; Pseudocholinesterase; BChE |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody selectively captures completely denatured human butyrylcholinesterase. The antibody does not bind native butyrylcholinesterase. The antibody does not recognise mouse butyrylcholinesterase. |
| Application Notes: | The authors developed an antibody that can be used for immunopurifying heat-inactivated, denatured human butyrylcholinesterase. The specificity of the antibody (IgG1 format) for denatured butyrylcholinesterase was confirmed by ELISA analysis.The immobilized antibody was able to selectively extracts denatured butyrylcholinesterase from plasma. The selective removal of completely denatured butyrylcholinesterase by the immobilized antibody was visualized on Western blots. Mass spectrometry confirmed the finding from Western blots that the antibody immunoputifies butyrylcholinesterase from heat-inactivated plasma (Peng et al., 2016; PMID: 28058292). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |