Mfg Part Number
Ab03088-10.0
Vector Labs Ab03088-10.0 Anti-HLA-A1:MAGE-A1 complex [HYB3], Human IgG1, lambda (200 μg)
Quick Overview
This antibody was raised by panning against the L chain-shuffling library raised by cloning the G8 VH gene into a vector containing a library of human Ab kappa and lambda L chains.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | HYB3 |
| Synonyms: | human leukocyte antigen (HLA)/peptide complex HLA-A1·MAGE-A1; tumor-associated HLA-A1·melanoma-associated antigen (MAGE)-A1 complex |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted human antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody is specific for human HLA-A1:MAGE-A1 complex. |
| Application Notes: | The structure of the fab version of this antibody in complex with HLA-A1 and MAGE-A1 was eludicated using X-ray crystallography. This was done to determine how the antibody binds to its ligand (Hülsmeyer et al, 2005; pmid:15537658). To characterize this antibody flow cytometry was preformed. Flow cytometry was preformed with the fab version of this antibody on dendritic cells (Chames et al, 2002; pmid:12097420). To assess the expression of low and high affinity scFv-y CD28 and scFv- receptors on gene-transduced primary human T lymphocytes. Flow cytometry was preformed on gene-transduced primary human T lymphocytes using the scFv version of this antibody. Furthermore a cytotoxic assay was preformed on scFv-y + CD28 or scFv-transduced human T lymphocytes. The cytotoxic assay was preformed using Cr51 release assay (Willemsen et al, 2005; pmid:15944290). To characterize this antibody after expressing the antibody in E.coli. An ELISA was preformed using the fab version of this antibody on a biotinylated M1/A1 complex. Furthermore to asses the ability of this antibody to bind M1/A1-positive cells. Flow cytometry was preformed on these cells using the scFv version of this antibody (Saeed et al, 2016; pmid:27022262). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |