Mfg Part Number
Ab03097-10.0
Vector Labs Ab03097-10.0 Anti-NSE [E2-4F6], Human IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with a peptide comprising 285-298aa (RDYPVVSIEDPFDQ) of human NSE protein cross linked with keyhole limpethemacyanin (KLH) mixed with an equal volume of complete Freund’s adjuvant (CFA) .
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | E2-4F6 |
| Synonyms: | ENO2; Neuron specific enolase; Gamma enolase; Enolase 2; Neural enolase; 2-phospho-D-glycerate hydro-lyase; E2; 4F6; NSE-E2 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody reacts with amino acids 285-298aa (RDYPVVSIEDPFDQ) of human NSE protein. Neuron specific enolase has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. It is also reported to bind cultured neocortical neurons in a calcium dependent manner and promotes cell survival. |
| Application Notes: | This antibody in combination with another anti-NSE antibody E1-7E2 from our catalogue can be used in a sandwich ELISA for detection of human gamma enolase in a sample. This antibody was also used as a primary antibody in the detection of NSE protein in A549 cells using western blot (CN111234023). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |