Mfg Part Number
Ab03098-10.3
Vector Labs Ab03098-10.3 Anti-TREM-1 [14F69], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing both wild type BALB/c mice and TREM-1 knock-out (KO) mice (C57BL/6 background) with either human TREM-1, cells expressing hTREM-1 (BWZ.36 cells), or a combination of both.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 14F69 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | TREM1; CD354; Triggering receptor expressed on myeloid cells 1; Triggering receptor expressed on monocytes 1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; Block |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds human, cynomolgus monkey and rhesus monkey TREM-1. TREM1 is a receptor that is expressed on monocytes, macrophages, neutrophils and dendritic cells. It plays important roles in innate and adaptive immunity by amplifying inflammatory responses. When activated, TREM-1 associates with the ITAM-containing signaling adaptor protein, DAP12. Downstream signalling may include activation of the NFAT transcription factor, causing an upregulation of pro-inflammatory cytokine production. |
| Application Notes: | The original IgG2a antibody binds human TREM-1 with an affinity of 2 X 10^-10 nM as tested by suface plasmon resonance. The screening of original antibody for binding human TREM-1 was done using ELISA and flow cytometry. This antibody is capable of binding both human, cynomolgus monkey and rhesus monkey PBMCs. This antibody was reported to block the release of TNFalpha from stimulated peripheral blood mononuclear cells (PBMCs). This antibody was also able to block the ligand-induced TREM-1 activation in such BWZ/hTREM-1 reporter cell assay (US9000127). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |