Mfg Part Number
Ab03099-10.3
Vector Labs Ab03099-10.3 Anti-TREM-1 [14F128], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing both wild type BALB/c mice and TREM-1 knock-out (KO) mice (C57BL/6 background) with either human TREM-1, cells expressing hTREM-1 (BWZ.36 cells), or a combination of both.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 14F128 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | TREM1; CD354; Triggering receptor expressed on myeloid cells 1; Triggering receptor expressed on monocytes 1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds human, cynomolgus monkey and rhesus monkey TREM-1. TREM1 is a receptor that is expressed on monocytes, macrophages, neutrophils and dendritic cells. It plays important roles in innate and adaptive immunity by amplifying inflammatory responses. When activated, TREM-1 associates with the ITAM-containing signaling adaptor protein, DAP12. Downstream signalling may include activation of the NFAT transcription factor, causing an upregulation of pro-inflammatory cytokine production. |
| Application Notes: | The original IgG1 antibody binds human TREM-1 with an affinity of 2.4 nM as tested by suface plasmon resonance. The screening of original antibody for binding human TREM-1 was done using ELISA and flow cytometry. This antibody is capable of binding both human, cynomolgus monkey and rhesus monkey PBMCs. This antibody was able to reduce activation of BWZ/hTREM-1 reporter cell. This antibody was also able to block the ligand-induced TREM-1 activation in such BWZ/hTREM-1 reporter cell assay (US9000127). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |