Vector Labs Ab03103-10.0 Anti-CD47 [ZF1], Human IgG1, lambda (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: ZF1
Synonyms: IAP; MER6; OV-3; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; Protein MER6; ovarian cancer marker
Amount: 200 μg
Application Codes Clone: ELISA; FC; Block
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds the extracellular domain of the human CD47, which is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epithelia and the brain. IAP or integrin assocaited protein has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It further plays an important role in memory formation and synaptic plasticity in the hippocampus. It also acts as a receptor for SIRPA. Binding to SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Application Notes: This antibody displayed high specificity and affinity for CD47 protein, which were comparable to those for humanized anti-CD47 blocking antibody B6H12. This antibody is could efficiently block the physical interaction of immobilized recombinant human CD47 to human and mouse SIRPα in blocking assay in vitro and was capable of inducing in vitro macrophage-mediated phagocytosis as robust as B6H12. It binds recombinant human CD47 in a dose dependent manner in an ELISA. This antibody was also capable of recognizing cell-surface expressed CD47 in a flow cytometry. The binding affinity of the IgG version of this antibody toward CD47 was determined to be 3.50 ± 0.16 nM using surface plasmon resonance. ZF1 treatment could induce robust phagocytosis of leukemic cancer cells by macrophage in vitro, and protect BALB/c nude mice from cancer killing by engrafted leukemic cells (CCRF and U937) to a similar extent as humanized B6H12 did (PMID: 27863402).

Product Class:	Purified
Clone ID:	ZF1
Synonyms:	IAP; MER6; OV-3; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; Protein MER6; ovarian cancer marker
Amount:	200 μg
Application Codes Clone:	ELISA; FC; Block
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds the extracellular domain of the human CD47, which is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epithelia and the brain. IAP or integrin assocaited protein has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It further plays an important role in memory formation and synaptic plasticity in the hippocampus. It also acts as a receptor for SIRPA. Binding to SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Application Notes:	This antibody displayed high specificity and affinity for CD47 protein, which were comparable to those for humanized anti-CD47 blocking antibody B6H12. This antibody is could efficiently block the physical interaction of immobilized recombinant human CD47 to human and mouse SIRPα in blocking assay in vitro and was capable of inducing in vitro macrophage-mediated phagocytosis as robust as B6H12. It binds recombinant human CD47 in a dose dependent manner in an ELISA. This antibody was also capable of recognizing cell-surface expressed CD47 in a flow cytometry. The binding affinity of the IgG version of this antibody toward CD47 was determined to be 3.50 ± 0.16 nM using surface plasmon resonance. ZF1 treatment could induce robust phagocytosis of leukemic cancer cells by macrophage in vitro, and protect BALB/c nude mice from cancer killing by engrafted leukemic cells (CCRF and U937) to a similar extent as humanized B6H12 did (PMID: 27863402).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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