Vector Labs Ab03104-23.0 Anti-CD47 [h4C1], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: h4C1
Synonyms: IAP; MER6; OV-3; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; Protein MER6; ovarian cancer marker; 4C1
Amount: 200 μg
Application Codes Clone: ELISA; FC; Block
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds the FG loop of the human CD47, which interacts with SIRPα and several other known CD47/SIRPα-blocking mAb. CD47 is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epithelia and the brain. IAP or integrin assocaited protein has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It further plays an important role in memory formation and synaptic plasticity in the hippocampus. It also acts as a receptor for SIRPA. Binding to SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Application Notes: This antibody specifically binds to the CD47 molecule to block the binding of CD47 to SIRPα, thereby activating macrophages and killing tumor cells. The blocking activity was demonstrated using flow cytometry assay. This antibody also bound CD47 ectodomain in an ELISA. Surface plasmon resonance (SPR)-binding assays demonstrated that h4C1 bound to the human CD47-ECD antigen with a KD of 0.85 nM. Macrophage-mediated phagocytosis activity was investigated by a tumor cell phagocytosis assay with bone marrow-derived macrophages (BMDMs) and tt was reported that h4C1 exhibited significant phagocytosis in Raji cells. In in vivo experiments this antibody improved survival and enhanced suppression of Raji cell xenografts in mice (PMID: 32296041). This antibody was also used in the generation of a bispecific antibody (BsAb) in a dual-variable-domain immunoglobulin (DVD-Ig) format targeting CD47 and PD-L1. The variable-domain from the PD-L1-blocking mAb #18 was introduced in tandem onto h4C1 to generate a dual variable domain bsAb that binds bivalently to both CD47 and PD-L1. This BsAb retained retained blocking activity for both interactions but lacked the hemagglutinating activity of the parental m4C1 (PMID: 32296041; 33244513).

Product Class:	Purified
Clone ID:	h4C1
Synonyms:	IAP; MER6; OV-3; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; Protein MER6; ovarian cancer marker; 4C1
Amount:	200 μg
Application Codes Clone:	ELISA; FC; Block
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds the FG loop of the human CD47, which interacts with SIRPα and several other known CD47/SIRPα-blocking mAb. CD47 is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epithelia and the brain. IAP or integrin assocaited protein has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It further plays an important role in memory formation and synaptic plasticity in the hippocampus. It also acts as a receptor for SIRPA. Binding to SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Application Notes:	This antibody specifically binds to the CD47 molecule to block the binding of CD47 to SIRPα, thereby activating macrophages and killing tumor cells. The blocking activity was demonstrated using flow cytometry assay. This antibody also bound CD47 ectodomain in an ELISA. Surface plasmon resonance (SPR)-binding assays demonstrated that h4C1 bound to the human CD47-ECD antigen with a KD of 0.85 nM. Macrophage-mediated phagocytosis activity was investigated by a tumor cell phagocytosis assay with bone marrow-derived macrophages (BMDMs) and tt was reported that h4C1 exhibited significant phagocytosis in Raji cells. In in vivo experiments this antibody improved survival and enhanced suppression of Raji cell xenografts in mice (PMID: 32296041). This antibody was also used in the generation of a bispecific antibody (BsAb) in a dual-variable-domain immunoglobulin (DVD-Ig) format targeting CD47 and PD-L1. The variable-domain from the PD-L1-blocking mAb #18 was introduced in tandem onto h4C1 to generate a dual variable domain bsAb that binds bivalently to both CD47 and PD-L1. This BsAb retained retained blocking activity for both interactions but lacked the hemagglutinating activity of the parental m4C1 (PMID: 32296041; 33244513).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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