Vector Labs Ab03106-23.0 Anti-Melanotransferrin [SC57.32], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: SC57.32
Synonyms: Melanoma-associated antigen p97; CD228; MTf; MFI2
Amount: 200 μg
Application Codes Clone: Crystallography; SPR; IHC; in vitro; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The Fab fragment is specific for human melanotransferrin (MTf). In particular, it targets the C2 subdomain of the MTf C-lobe, making direct contact with ten MTf residues (Arg 458, Arg 459, Asp 460, Ser 461, His 463, Arg 474, Val 564, Glu 565, Asn 566, Asp 598) as well as two N-acetylglucosamines of the Asn 515 N-glycan. The Fab fragment cross reacts with cynomolgus monkey MTf but not with rat and mouse MTf. Melanotransferrin is a 75 kDa member of the transferrin superfamily, which comprises a single chain, iron-binding glycoproteins responsible for iron homeostasis in cells.
Application Notes: The crystal structure of the Fab fragment in complex with human melanotransferrin (hMFt) was determined. Surface plasmon resonance assay was performed to evaluate the binding specificity and affinity between the Fab fragment and hMFt (Kd= 1.0 nM). The Fab fragment is the murine version of SC-005, which has been used in clinical trials (Hayashi et al., 2021; PMID: 33436675). Immunohistochemistry was performed on HEK293T parental cell pellets or MFI2-expressing HEK293T cell pellets using the murine version of the antibody, which was able to specifically detect MFI2-overexpressing HEK293T cell pellets. The humanized version of the antibody was able to kill HEK-293T cells overexpressing MFI2, showing comparable efficacy to the chimeric antibody. In vivo studies with the humanized version of the antibody conjugated to a pyrrolobenzodiazepine resulted in significant tumor suppression in mice (US10428156).

Product Class:	Purified
Clone ID:	SC57.32
Synonyms:	Melanoma-associated antigen p97; CD228; MTf; MFI2
Amount:	200 μg
Application Codes Clone:	Crystallography; SPR; IHC; in vitro; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The Fab fragment is specific for human melanotransferrin (MTf). In particular, it targets the C2 subdomain of the MTf C-lobe, making direct contact with ten MTf residues (Arg 458, Arg 459, Asp 460, Ser 461, His 463, Arg 474, Val 564, Glu 565, Asn 566, Asp 598) as well as two N-acetylglucosamines of the Asn 515 N-glycan. The Fab fragment cross reacts with cynomolgus monkey MTf but not with rat and mouse MTf. Melanotransferrin is a 75 kDa member of the transferrin superfamily, which comprises a single chain, iron-binding glycoproteins responsible for iron homeostasis in cells.
Application Notes:	The crystal structure of the Fab fragment in complex with human melanotransferrin (hMFt) was determined. Surface plasmon resonance assay was performed to evaluate the binding specificity and affinity between the Fab fragment and hMFt (Kd= 1.0 nM). The Fab fragment is the murine version of SC-005, which has been used in clinical trials (Hayashi et al., 2021; PMID: 33436675). Immunohistochemistry was performed on HEK293T parental cell pellets or MFI2-expressing HEK293T cell pellets using the murine version of the antibody, which was able to specifically detect MFI2-overexpressing HEK293T cell pellets. The humanized version of the antibody was able to kill HEK-293T cells overexpressing MFI2, showing comparable efficacy to the chimeric antibody. In vivo studies with the humanized version of the antibody conjugated to a pyrrolobenzodiazepine resulted in significant tumor suppression in mice (US10428156).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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