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Mfg Part Number

Ab03107-1.1

Vector Labs Ab03107-1.1 Anti-Diethoxyphosphorothioate [12C2], Mouse IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The antibody was generated by immunizing BABL/c mice with the diethoxyphosphorothioate derivated hapten, followed by antibody library construction and phage display-based selection.

$517.40

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Description

Product Class: Purified
Clone ID: 12C2
Synonyms: O,O-diethyl organophosphorus pesticides; DOPs; OPs
Amount: 200 μg
Application Codes Clone: WB; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody is specific for a broad set of O,O-diethyl organophosphorus pesticides (DOPs). DOPs are widely used for agricultural and landscape pest control. However, it has been reported that OPs are harmful to human health though food exposure.
Application Notes: The specificity of this antibody for a broad set of O,O-diethyl organophosphorus pesticides (DOPs) was confirmed by indirect competitive ELISA. IC50 of the Fab antibody was 135.7ng/mL. A standard curve showed that the linear detection range is 7.2ng/mL-861.7ng/mL. The Fab fragment detected soluble DOPs by western blot (Chen et al., 2018;PMID: 29734787). The specificity of the original format of the antibody was confirmed by competitive indirect ELISA analysis (Xu et al. 2010; PMID: 20958019). The original format of the antibody was linked to alkaline phosphatase (scFv−AP) and employed to develop a competitive direct enzyme-linked immunosorbent assay (cdELISA) for O,O-diethyl OPs in environmental and agricultural samples (Xu et al. 2012; PMID: 22533788).

Product Class:	Purified
Clone ID:	12C2
Synonyms:	O,O-diethyl organophosphorus pesticides; DOPs; OPs
Amount:	200 μg
Application Codes Clone:	WB; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody is specific for a broad set of O,O-diethyl organophosphorus pesticides (DOPs). DOPs are widely used for agricultural and landscape pest control. However, it has been reported that OPs are harmful to human health though food exposure.
Application Notes:	The specificity of this antibody for a broad set of O,O-diethyl organophosphorus pesticides (DOPs) was confirmed by indirect competitive ELISA. IC50 of the Fab antibody was 135.7ng/mL. A standard curve showed that the linear detection range is 7.2ng/mL-861.7ng/mL. The Fab fragment detected soluble DOPs by western blot (Chen et al., 2018;PMID: 29734787). The specificity of the original format of the antibody was confirmed by competitive indirect ELISA analysis (Xu et al. 2010; PMID: 20958019). The original format of the antibody was linked to alkaline phosphatase (scFv−AP) and employed to develop a competitive direct enzyme-linked immunosorbent assay (cdELISA) for O,O-diethyl OPs in environmental and agricultural samples (Xu et al. 2012; PMID: 22533788).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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