Mfg Part Number
Ab03107-23.0
Vector Labs Ab03107-23.0 Anti-Diethoxyphosphorothioate [12C2], Rabbit IgG, kappa (200 μg)
Quick Overview
The antibody was generated by immunizing BABL/c mice with the diethoxyphosphorothioate derivated hapten, followed by antibody library construction and phage display-based selection.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 12C2 |
| Synonyms: | O,O-diethyl organophosphorus pesticides; DOPs; OPs |
| Amount: | 200 μg |
| Application Codes Clone: | WB; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse Fab format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody is specific for a broad set of O,O-diethyl organophosphorus pesticides (DOPs). DOPs are widely used for agricultural and landscape pest control. However, it has been reported that OPs are harmful to human health though food exposure. |
| Application Notes: | The specificity of this antibody for a broad set of O,O-diethyl organophosphorus pesticides (DOPs) was confirmed by indirect competitive ELISA. IC50 of the Fab antibody was 135.7ng/mL. A standard curve showed that the linear detection range is 7.2ng/mL-861.7ng/mL. The Fab fragment detected soluble DOPs by western blot (Chen et al., 2018;PMID: 29734787). The specificity of the original format of the antibody was confirmed by competitive indirect ELISA analysis (Xu et al. 2010; PMID: 20958019). The original format of the antibody was linked to alkaline phosphatase (scFv−AP) and employed to develop a competitive direct enzyme-linked immunosorbent assay (cdELISA) for O,O-diethyl OPs in environmental and agricultural samples (Xu et al. 2012; PMID: 22533788). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |