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Mfg Part Number

Ab03108-3.0

Vector Labs Ab03108-3.0 Anti-FimA [123-123-10], Mouse IgG2b, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing BALB/c mice with FimA protein.

$517.40

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Description

Product Class: Purified
Clone ID: 123-123-10
Synonyms: Major fimbrium subunit FimA type-1; Fimbrillin; Fimbrilin; Major fimbrial subunit protein type I; fimbrial protein; fimbriae protein
Amount: 200 μg
Application Codes Clone: ELISA; WB; inhibition
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: The antibody reacts with conformational epitopes displayed by partially dissociated oligomers of FimA, but not with the 43 kDa FimA monomer. FimA is a structural protein of P. gingivalis fimbriae and is known to be involved in bacterial adherence to the host cell surface.
Application Notes: The antibody reacted with conformational epitopes expressed by partially dissociated oligomers, but not with monomer as elucidated by Western blot analysis. Ascites fluid containing the antibody showed the inhibitory activity against P. gingivalis to saliva-coated hydroxyapatite beads, an in vitro model for the pellicle-coated tooth surface. For example, 400-fold diluted antibody inhibited the binding of the bacteria onto bead surfaces of 29.5% (Koh et al., 2009; PMID: 20157608). The specificity of this antibody (IgG2b) was confirmed by ELISA analysis. The original antibody detected FimA by western blot. The antibody significantly inhibited the binding of P. gingivalis to the surface of saliva-coated hydroxyapatite (SHA) beads by 49.79 ± 5.0%, when 50 ng/mL antibody was used (P < 0.01). The antibody could be used as a basis for passive immunization against P. gingivalis-mediated periodontitis (Koh et al., 2011; PMID: 21223367).

Product Class:	Purified
Clone ID:	123-123-10
Synonyms:	Major fimbrium subunit FimA type-1; Fimbrillin; Fimbrilin; Major fimbrial subunit protein type I; fimbrial protein; fimbriae protein
Amount:	200 μg
Application Codes Clone:	ELISA; WB; inhibition
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	The antibody reacts with conformational epitopes displayed by partially dissociated oligomers of FimA, but not with the 43 kDa FimA monomer. FimA is a structural protein of P. gingivalis fimbriae and is known to be involved in bacterial adherence to the host cell surface.
Application Notes:	The antibody reacted with conformational epitopes expressed by partially dissociated oligomers, but not with monomer as elucidated by Western blot analysis. Ascites fluid containing the antibody showed the inhibitory activity against P. gingivalis to saliva-coated hydroxyapatite beads, an in vitro model for the pellicle-coated tooth surface. For example, 400-fold diluted antibody inhibited the binding of the bacteria onto bead surfaces of 29.5% (Koh et al., 2009; PMID: 20157608). The specificity of this antibody (IgG2b) was confirmed by ELISA analysis. The original antibody detected FimA by western blot. The antibody significantly inhibited the binding of P. gingivalis to the surface of saliva-coated hydroxyapatite (SHA) beads by 49.79 ± 5.0%, when 50 ng/mL antibody was used (P < 0.01). The antibody could be used as a basis for passive immunization against P. gingivalis-mediated periodontitis (Koh et al., 2011; PMID: 21223367).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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